摘要
Abstract
To investigate the effects of high-concentrate diet on growth performance,serum biochemical indices and intestinal microflora structure of weaned fattening lambs,a total of 20 healthy weaned male crossbred lambs(Dorper ♂×Hu sheep ♀)were randomly divided into control group and experimental group,with 10 lambs each group.The experimental group was fed a high-concentrate diet with a concentrate-to-roughage ratio of 75∶25,and the control group was fed a conventional fattening diet with a concentrate-to-roughage ratio of 50∶50.The experimental period lasted 60 days.Growth performance of lambs、serum biochemical indices were measured and intestinal microflora was analyzed by 16S rRNA sequencing.The results showed that:①Compared with control group,the final body weight,total weight gain and average daily gain of lambs in experimental group were significantly increased(P<0.05),and the feed-to-gain ratio was significantly decreased(P<0.05).②The contents of serum total protein(TP),urea(UREA)and creatinine(CREA)in experimental group were extremely significantly higher than those in control group(P<0.01);the content of total bilirubin(TBIL)in experimental group was significantly lower than that in control group(P<0.05);the contents of potassium(K)and phosphorus(P)in serum of experimental group were significantly lower than those in control group(P<0.05).③ At the phylum level of intestinal microbiota,the relative abundances of Firmicutes-C and Firmicutes-A in the experimental group were extremely significantly and significantly lower than those in the control group(P<0.01 or P<0.05),respectively.The relative abundances of Fibrobacterota and Basidiomycota were extremely significantly higher in the experimental group(P<0.01 or P<0.05).At the genus level,the experimental group showed extremely significantly and significantly higher abundances of Fibrobacter,Naganishia,Paraprevotella and Fusarium compared with the control group(P<0.01 or P<0.05).The abundance of Microascus was extremely significantly lower in the experimental group(P<0.01).Functional prediction of intestinal microflora showed that the highly enriched pathways included amino acid metabolism,carbohydrate metabolism and metabolism of cofactors and vitamins.In conclusion,feeding high-concentrate diets could significantly improve the growth performance of weaned fattening lambs,ameliorate the body metabolic status,and optimize the intestinal microecological structure.关键词
断奶羔羊/高精料育肥/生长性能/血清指标/肠道菌群Key words
weaned lambs/high-concentrate fattening/growth performance/serum indices/intestinal microflora分类
农业科技
