华侨大学学报（自然科学版）2026，Vol.47Issue(4)：473-481,9.DOI:10.11830/ISSN.1000-5013.202507033
高产透明质酸兽疫链球菌的诱变选育与发酵优化及代谢组学分析
Mutagenesis Breeding,Fermentation Optimization and Metabolomic Analysis of Streptococcus zooepidemicus With High-Yield Hyaluronic Acid
摘要
Abstract
In order to enhance hyaluronic acid yield of Streptococcus zooepidemicus and investigate its meta-bolic mechanisms under varying fermentation conditions,S.zooepidemicus ATCC 39920 was subjected to compound mutagenesis using ultraviolet and diethyl sulfate to screen a high-yield hyaluronic acid mutant strain SQ49,its fermentation conditions in 5 L bioreactors were optimized,and the intracellular differential metabo-lites under varying temperature and initial glucose concentration conditions were analyzed using metabolomics.The results showed that the hyaluronic acid yield of SQ49 reached 1.05 g·L-1,2.83 times higher than that of its parent strain.Under the optimal fermentation conditions(ventilation capacity of 12 L·min-1,agitation speed of 600 r·min-1,pH value at 7.5,initial glucose mass concentration of 60 g·L-1,and temperature at 37℃),the hyaluronic acid yield of SQ49 was increased to 6.61 g·L-1.Metabolomics results showed that the accumulation of DL-leucyl-valine was a key temperature-controlled target driving high hyaluronic acid yield,and accumulating palmitic acid and inhibiting lactic acid accumulation were key carbon source-controlled targets for enhancing hyaluronic acid yield.关键词
透明质酸/发酵条件优化/菌种选育/代谢组学Key words
hyaluronic acid/fermentation condition optimization/strain breeding/metabolomics分类
化学化工引用本文复制引用
陶炳毅,张永莉,何仁,梁少雄,黄婷亭,陈鸿坤,饶小平,汤须崇..高产透明质酸兽疫链球菌的诱变选育与发酵优化及代谢组学分析[J].华侨大学学报（自然科学版）,2026,47(4):473-481,9.基金项目
福建省厦门市未来产业领域重点科技计划项目(3502Z20254020) （3502Z20254020）
福建省厦门市高校科研院所产学研项目(2024CXY0217) （2024CXY0217）
福建省南平市科技计划项目(N2023Y015) （N2023Y015）
