湖南农业科学Issue(4)：46-53,8.DOI:10.16498/j.cnki.hnnykx.2026.004.008
魔芋软腐病抗感品种微生物群落组成与多样性分析
Microbial Community Composition and Diversity of Resistant and Susceptible Konjac Species to Soft Rot
摘要
Abstract
To elucidate the potential relationship between the endophytic microbial community composition in petiole sap and resistance to konjac soft rot,using resistant species Amorphophallus muelleri and susceptible species Amorphophallus konjac as materials for soft rot Illumina MiSeq high-throughput sequencing was employed to analyze the bacterial and fungal community structures and diversity in the petiole sap of resistant and susceptible konjac species.The results showed that the disease-resistant A.muelleri had higher total number of bacterial OTUs and number of unique bacterial OTUs but lower total number of fungal OTUs and number of unique fungal OTUs than the susceptible A.konjac.Additionally,A.muelleri had higher Chao1 index and Shannon index of bacteria and Shannon index of fungi than A.konjac.Hierarchical clustering and principal coordinate analysis revealed that resistant and susceptible konjac samples were clustered into distinct groups,indicating significant differences in microbial community composition in their petiole sap.At the phylum level,bacterial communities in both resistant and susceptible konjac species were primarily dominated by Proteobacteria,Actinobacteria,and Firmicutes,while fungal communities were mainly composed of Ascomycota and Basidiomycota.A.muelleri had higher relative abundance of Ascomycota(81.25%vs.47.00%)but lower abundance of Basidiomycota(18.00%vs.35.47%)than A.konjac.At the genus level,A.muelleri showed significantly higher relative abundance of beneficial microbial genera(including Sphingomonas,Pseudomonas,Rhizobium,and Microbacterium)but significantly lower relative abundance of potentially pathogenic genera(such as Erwinia and Filobasidium)than A.konjac.The above results indicated significant differences in the composition and relative abundance of microbial communities in the petiole sap between susceptible A.konjac and resistant A.muelleri.This suggested a potential link between the endophytic microbial community composition and the resistance to konjac soft rot.关键词
珠芽金魔芋/花魔芋/软腐病/内生微生物/细菌/真菌Key words
Amorphophallus muelleri/Amorphophallus konjac/soft rot/endophytic microorganism/bacterium/fungus分类
农业科技引用本文复制引用
陈凯,刘佳妮,杨敏,王维,唐兰,李丽芳,赵永腾,高鹏华,齐颖,赵建荣,余磊..魔芋软腐病抗感品种微生物群落组成与多样性分析[J].湖南农业科学,2026,(4):46-53,8.基金项目
云南省科技厅云南省魔芋生物学重点实验室专项(202449CE340009) （202449CE340009）
云南省魔芋资源保护与利用国际联合实验室专项(202503AP140005) （202503AP140005）
云南省科技厅科技计划项目(202501AU070008) （202501AU070008）
云南省教育厅科研项目(FWCY-QYCT2025017,2025J0753) （FWCY-QYCT2025017,2025J0753）
云南省余磊专家基层科研工作站(20231023-135) （20231023-135）
