家畜生态学报2026，Vol.47Issue(6)：18-24,7.DOI:10.3969/j.issn.1673-1182.2026.06.003
α-亚麻酸对猪卵丘卵母细胞转录组的影响
Effects of α-Linolenic Acid on the Transcriptome of Porcine Cumulus-Oocyte Complexes
摘要
Abstract
To investigate the mechanisms by which alpha-linolenic acid(ALA)promotes meiotic matu-ration and cumulus-oocyte interactions in porcine oocytes,this study employed an experimental design in which ALA was added to the culture medium of cumulus-oocyte complexes(COCs),with a non-supple-mented group serving as the control.Cumulus cells and oocytes were collected separately for transcriptome sequencing analysis,with three biological replicates per group.Differentially expressed genes(DEGs)were identified using DESeq2,followed by GO and KEGG enrichment analyses,clustering,and protein-protein interaction network analysis.The results revealed that ALA treatment led to the significant upregulation of 42 genes and downregulation of 38 genes in oocytes(P≤0.05).In granulosa cells,37 genes were signifi-cantly upregulated and 100 downregulated following ALA treatment(P≤0.05).In granulosa cells,DEGs were primarily enriched in pathways related to glycolysis/glucose transport and lipid metabolism,whereas in oocytes,DEGs were associated with actin filament organization and the extracellular space.Interaction analysis of key regulatory genes involved in pathways commonly enriched in both cumulus cells and oocytes highlighted a high frequency of interactions among genes in the cAMP signaling pathway.These findings provide essential foundational data for further exploration of the mechanisms by which ALA facilitates in vitro oocyte maturation and modulates cumulus-oocyte communication.关键词
猪卵母细胞/体外成熟/α-亚麻酸/转录组/卵丘卵母细胞互作Key words
porcine oocytes/in vitro maturation/Alpha-linolenic acid/transcriptome/cumulus-oocyte in-teraction分类
农业科技引用本文复制引用
石长威,许豪泽,赵嘉艺,高磊..α-亚麻酸对猪卵丘卵母细胞转录组的影响[J].家畜生态学报,2026,47(6):18-24,7.基金项目
国家自然科学基金青年科学基金项目(32102548) （32102548）
国家生猪产业技术体系(CARS-35-PIG) （CARS-35-PIG）
