基础医学与临床2026，Vol.46Issue(7)：982-989,8.DOI:10.16352/j.issn.1001-6325.2026.07.0982
富含AU元件(AREs)及其结合蛋白协同发育程序
AU-rich elements(AREs)and their binding proteins coordinate developmental programs
辛明君 1霍悦 1王芳1
作者信息
- 1. 中国医学科学院北京协和医学院 基础医学研究所 重大疾病共性机制研究国家重点实验室,北京 100005
- 折叠
摘要
Abstract
Objective To explore the potential synergistic roles of AU-rich elements(AREs)and their binding proteins(AREBPs)in post-transcriptional regulation and the translation process.Methods A customized algorithm was utilized to extract the core ARE motifs within the 3′UTR regions across human and mouse genomes.The binding sites of AREBPs were identified by integrating data from the Postar3 database,public CLIP-seq data-sets from GEO.Independently constructed CLIP-seq database and PrismNet deep learning predictions.Fisher's exact test was applied to calculate the enrichment degree(odds ratio)of specific AREBPs in tissue-specific genes.The co-expression patterns between AREBPs and target genes were analyzed by combining the GTEx database and tis-sue-specific developmental RNA-seq datasets(e.g.,kidney development dataset GSE100859).Results A com-prehensive genome-wide map of conserved 3′UTR AREs in humans and mice was successfully generated.Specific AREBPs exhibited significant binding enrichment(P＜0.05)in tissue-specific genes associated with muscle,brain,kidney and heart.Expression profiling analysis revealed that during tissue development(such as kidney development),genes containing AREs showed significantly different expression patterns compared to those without AREs.Conclu-sions AREs and their binding proteins play a highly specific function in kidney development,genes containing AREs showed significantly different expression patterns as compared to those without AREs.Conclusions AREs and their binding proteins play a highly specific role in post-transcriptional regulation across different tissues.This study provides a crucial bioinformatics evidence for understanding the RNA regulatory mechanisms underlying tissue de-velopment and homeostasis maintenance.关键词
增强型紫外交联免疫共沉淀测序(eCLIP-seq)/富含腺嘌呤尿嘧啶元件(AREs)/组织发育/RNA结合蛋白/多组学/数据库Key words
enhanced UV crosslinking immunoprecipitation-sequencing(eCLIP-seq)/AU-rich elements(AREs)/tissue develop-ment/RNA-binding proteins/multi-omics/database分类
生物科学引用本文复制引用
辛明君,霍悦,王芳..富含AU元件(AREs)及其结合蛋白协同发育程序[J].基础医学与临床,2026,46(7):982-989,8.
