经济动物学报2026，Vol.30Issue(3)：220-234,15.DOI:10.13326/j.jea.2026.1994
CRISPR-Cas系统的类型、机制及应用进展
Diversified Types,Mechanisms and Application Progress of CRISPR-Cas System
摘要
Abstract
Biological evolution is mainly driven by random gene mutations and natural selection.The CRISPR-Cas system,as a novel gene-editing molecular tool,has achieved precise regulation of biological genetic directions.Owing to its advantages such as high targeting accuracy,simple operation,efficient editing,low cost,and compatibility with various technologies,this system shows broad application potential in multiple fields,including gene function analysis,gene-editing breeding,clinical treatment,and environmental governance.According to the differences in CRISPR sequence structural characteristics and Cas protein functions,there are mainly 2 classes and 6 types of CRISPR systems,among which the Cas3,Cas9,and Cas12 systems are the most widely used.This review summarizes the different types of CRISPR-Cas systems and the functional mechanisms of various Cas proteins,analyzes the current existing problems,and its development prospects,to provide a reference for research on the CRISPR-Cas system in fields such as gene editing and disease prevention.关键词
CRISPR/Cas蛋白/基因编辑/anti-CRISPRKey words
CRISPR/Cas protein/gene edit/anti-CRISPR分类
农业科技引用本文复制引用
卢泽宇,李蕴华,张传强,李媛,张令天,王浩源,刘佳森,赵艳红..CRISPR-Cas系统的类型、机制及应用进展[J].经济动物学报,2026,30(3):220-234,15.基金项目
国家自然科学基金项目(32160772),内蒙古自治区科技计划项目(2023YFHH0076),内蒙古自治区自然科学基金项目(2024ZD14),内蒙古自治区高等学校创新团队发展计划项目(NMGIRT2322) （32160772）
