江苏农业学报2026，Vol.42Issue(5)：963-972,10.DOI:10.3969/j.issn.1000-4440.2026.05.010
盐碱植物根际溶磷菌的筛选及对玉米生长的影响
Screening of phosphorus-solubilizing bacteria in the rhizosphere of saline-alkali plants and their effects on maize growth
摘要
Abstract
To further explore salt-tolerant and growth-promoting microbial resources and develop plant growth-promoting bacterial inoculants suitable for saline-alkali environments,this study employed selective media to isolate and screen phosphate-solubilizing bacteria from the rhizosphere soil of plants growing in saline-alkali grasslands in Kashi,Xinjiang.The taxonomic status of the strains was identified through 16S rRNA gene sequencing,and their growth-promoting effects on plants were evaluated using semi-solid tube plantlets and pot experiments with saline-alkali soil.A total of 19 phosphate-solubilizing bacterial strains were screened,with the amounts of solubilized phosphorus ranging from 7.67 μg/mL to 43.08 μg/mL.The strains c3-9,f1-8,b1-8 and c3-1 ranked among the top four in terms of phosphate-solubilizing amounts and exhibited a certain tolerance to saline-alkali stress.The 19 phosphate-solubilizing bacterial strains belonged to six genera,including Enterobacter,Acinetobacter,Pseudomonas,Bacillus,Chryseomonas,and Leclercia.Inoculation with Pseudomonas strain f1-8,Acinetobacter strain b1-8,Enterobacter hormaechei strain c3-1,and Leclercia strain c3-9 all promoted the growth of maize plantlets under non-saline-alkali stress to varying degrees.Pot experiments with saline-alkali soil showed that,compared with the control,strain f1-8 significantly increased maize plant height and above-ground fresh weight(P＜0.05);strain c3-9 significantly promoted maize root dry weight(P＜0.05);strains f1-8 and c3-1 significantly affected total root volume(P＜0.05);and strains f1-8,b1-8,and c3-9 significantly influenced total root length(P＜0.05).Additionally,the physicochemical property analysis of the potting soil indicated that the available phosphorus content increased by 16.03％to 66.79％after inoculation with phosphate-solubilizing bacterial strains.The principal component analysis(PCA)results for maize growth indices and soil available phosphorus content indicated that the c3-9 treatment exhibited the best overall effect.These findings expand the germplasm resource library of phosphate-solubilizing bacteria for saline-alkali soils and provide a theoretical basis for the development of microbial fertilizers for saline-alkali soil improvement.关键词
盐碱地/溶磷菌/促进生长/玉米Key words
saline-alkali soil/phosphate-solubilizing bacteria/growth promotion/maize分类
农业科技引用本文复制引用
古鸣,李明源,李云霞,王继莲..盐碱植物根际溶磷菌的筛选及对玉米生长的影响[J].江苏农业学报,2026,42(5):963-972,10.基金项目
喀什地区科技计划项目(KS2024001) （KS2024001）
国家自然科学基金项目(32160408) （32160408）
