江苏农业学报2026，Vol.42Issue(5)：990-1003,14.DOI:10.3969/j.issn.1000-4440.2026.05.013
四川麻兔不同泌乳时期乳脂球膜蛋白的差异分析
Differential analysis of milk fat globule membrane proteins across lactation stages in Sichuan rabbits
摘要
Abstract
Milk fat globule membrane(MFGM)proteins play essential roles in lipid metabolism,immune defense,and the regulation of lactation.In this study,the asymmetric track lossless mass analyzer-data-independent acquisition(Astral-DIA)mass spectrometry platform was employed to profile the MFGM proteome of Sichuan rabbits across three lactation stages:early lactation(MM 1),mid-lactation(MM21),and late lactation(MM35).A total of 2 937 MFGM proteins were identified,of which 1 907 were consistently present throughout all lactation stages.Comparative analyses revealed 1 331 differentially expressed MFGM proteins(DEMPs)between early lactation and late lactation,458 DEMPs between mid-lactation and late lactation,and 1 351 DEMPs between early lactation and mid-lactation.Functional annotations based on KEGG pathway analysis,egg-NOG classification,Pfam domain identification,and subcellular localization prediction uncovered distinct stage-specific roles of MFGM proteins:those in early lactation were predominantly linked to immune function,while proteins in mid-lactation and late lactation were functionally stable and mainly involved in nutrient metabolism.Time-course expression patterns of characteristic MFGM proteins were further dissected using optimized Mfuzz clustering,and eight representative proteins were identified and functionally characterized.The MFGM proteins of Sichuan rabbits exhibited complex functional characteristics related to lipid metabolism,vesicle trafficking,signal transduction,and immune protection,supporting both energy supply and innate immunity in neonates.This study elucidates the specific proteomic characteristics and biological significance of MFGM proteins during lactation,providing an important foundation for subsequent research on the functional roles of MFGM proteins in milk secretion and development of newborn rabbit pups.关键词
四川麻兔/泌乳期/乳脂球膜蛋白Key words
Sichuan rabbits/lactation/milk fat globule membrane protein分类
农业科技引用本文复制引用
邝良德,向雨晗,付伟,李丛艳,王云端,李钰莹,刘艳,穆雪儿,雷岷,郑洁,郭志强,杨锐..四川麻兔不同泌乳时期乳脂球膜蛋白的差异分析[J].江苏农业学报,2026,42(5):990-1003,14.基金项目
四川省科研院所基本科研项目(SASA202405) （SASA202405）
国家现代农业产业技术体系四川兔创新团队项目(SCCXTD-2024-26) （SCCXTD-2024-26）
国家兔产业技术体系项目(CARS-43-D-1) （CARS-43-D-1）
四川省畜禽育种攻关项目(2021YFYZ0033) （2021YFYZ0033）
四川省财政运行专项(SASA2025CZYX004) （SASA2025CZYX004）
