扬州大学学报（农业与生命科学版）2026，Vol.47Issue(3)：44-50,7.DOI:10.16872/j.cnki.1671-4652.2026.03.006
基于GSK-3β/β-catenin通路探究健脾散结方对结肠癌大鼠肿瘤生长及炎症反应的影响
Exploration on effects of Jianpi Sanjie prescription on tumor growth and inflammatory response in rats with colon cancer based on GSK-3β/β-catenin pathways
摘要
Abstract
In order to explore the effects and mechanism of Jianpi Sanjie prescription on tumor growth and inflammato-ry response in rats with colon cancer,modes of colon cancer rat were induced by 1,2-dimethylhydrazine,and 48 Wistar rats were randomly divided into control group,model group,low-dose,medium-dose and high-dose Jianpi Sanjie prescrip-tion groups,and Jianpi Sanjie prescription+lithium chloride group,8 cases in each group.The changes of body mass were recorded,colon length and number of tumors were measured.The pathological changes of colon tissues were observed by HE staining,expression of proliferating cell nuclear antigen(PCNA)in colon tissues was detected by immunohisto-chemistry,levels of serum inflammatory factors were detected by enzyme-linked immunosorbent assay,and expressions of glycogen synthase kinase-3β/β-catenin(GSK-3β/β-catenin)pathway related proteins in colon tissues were detected by Western blotting.The results showed that compared with control group,body mass,colon length,levels of serum IL-10 and p-GSK-3β/GSK-3β in colon tissues were significantly decreased,number of tumors and PCNA-positive cells,levels of serum interleukin(IL-6,IL-1β)and tumor necrosis factor α(TNF-α),and β-catenin expression were significantly in-creased in model group(P＜0.05).Compared with model group,body mass,colon length levels of serum IL-10 and p-GSK-3β/GSK-3β in colon tissues were significantly increased,number of tumors and PCNA-positive cells,levels of IL-6,IL-1β and TNF-α,and β-catenin expression were significantly decreased in medium-dose and high-dose Jianpi Sanjie prescription groups(P＜0.05).Compared with high-dose Jianpi Sanjie prescription group,body mass,colon length,levels of serumIL-10 and p-GSK-3β/GSK-3β in colon tissues were significantly decreased,number of tumors and PCNA-positive cells,levels of IL-6,IL-1β and TNF-α,and β-catenin expression were significantly increased in Jianpi Sanjie prescription+lithi-um chloride group(P＜0.05).In conclusion,Jianpi Sanjie prescription may relieve inflammatory response by activating GSK-3β/β-catenin signaling pathways,and thus inhibit tumor growth in rats with colon cancer.关键词
大鼠/健脾散结方/结肠癌/肿瘤生长/炎症反应/糖原合成酶激酶-3β/β-连环蛋白信号通路Key words
rat/Jianpi Sanjie prescription/colon cancer/tumor growth/inflammatory response/GSK-3β/β-catenin signaling pathway分类
医药卫生引用本文复制引用
徐珩,李祥祥,曾永蕾,李学军,郝皖荣,丁艳琪,周先阳,马丽,吴勇,冷玉玲..基于GSK-3β/β-catenin通路探究健脾散结方对结肠癌大鼠肿瘤生长及炎症反应的影响[J].扬州大学学报（农业与生命科学版）,2026,47(3):44-50,7.基金项目
安徽省中医药传承创新项目(2020ccyb05) （2020ccyb05）
曾永蕾安徽省名医工作室项目[(2020)10号] （2020）
