扬州大学学报（农业与生命科学版）2026，Vol.47Issue(3)：51-60,10.DOI:10.16872/j.cnki.1671-4652.2026.03.007
运动减脂机制:自噬与炎症的关系及作用
Exploration of the mechanism of exercise for fat reduction:The relationship and role of autophagy and inflammation
摘要
Abstract
To observe how different exercise modalities affect autophagic activity and inflammatory status in visceral adipose tissue(VAT)of obese mice,and to clarify the autophagy-inflammation interplay after exercise so as to uncover potential molecular mechanisms of exercise-induced fat loss,170 4-week-old male C57BL/6 mice were fed a high-fat diet(HFD)to establish obesity.Forty-two mice that reached the obesity criterion were randomly divided into a high-fat diet sedentary control(HHC group,n=9),a normal-diet sedentary(HNC group,n=9),a normal-diet plus endurance exer-cise(HNE group,n=12),and a normal-diet plus resistance exercise(HNR group,n=12).HNE and HNR mice com-pleted 8 week of treadmill running or ladder climbing,respectively.VAT was then harvested.Autophagy-and inflamma-tion-related mRNAs and proteins were quantified by RT-PCR and Western blotting;autophagosomes and protein localiza-tion were visualized by transmission electron microscopy and immunohistochemistry.The results showed that:① Com-pared with the HHC group,the expression of Atg5 mRNA and the LC3 Ⅱ/LC3 Ⅰ ratio in the HNC group were signifi-cantly decreased,the expression of ULK1,Becn1,Atg5 mRNA in the HNE group was significantly decreased,the ex-pression of Atg7,p62 mRNA was significantly increased,the expression of Becn1 protein and the LC3 Ⅱ/LC3 Ⅰ ratio was significantly decreased,and the expression of p62 protein was significantly increased.In the HNR group,the expression of Atg5 mRNA was significantly decreased,the expression of Atg7,p62 mRNA was significantly increased,the expres-sion of Becn1 protein and the LC3 Ⅱ/LC3 Ⅰ ratio was significantly decreased;compared with the HNC group,the ex-pression of Atg7,p62 mRNA in the HNE group was significantly increased,the expression of p62 protein was signifi-cantly increased,and in the HNR group,the expression of Atg7 mRNA was significantly increased;compared with the HNE group,the expression of Becn1 mRNA in the HNR group was significantly increased,and the expression of p62 protein was significantly decreased.② Compared with the HHC group,the expression of IL-1β protein in the HNC group was significantly decreased,the expression of IL-10 mRNA,IL-6,IL-10 protein in the HNE group was significantly in-creased,the expression of IL-1β protein was significantly decreased,and the expression of IL-6,IL-10 mRNA and pro-tein in the HNR group was significantly increased,the expression of IL-1β protein was significantly decreased;compared with the HNC group,the expression of IL-10 mRNA in the HNE group was significantly increased,the expression of IL-6,IL-10 protein was significantly increased,the expression of IL-1β protein was significantly decreased,and the expression of IL-6,IL-10 mRNA and protein in the HNR group was significantly increased,the expression of IL-1β protein was sig-nificantly decreased;compared with the HNE group,the expression of IL-6 mRNA in the HNR group was significantly increased,and the expression of IL-10 mRNA was significantly decreased.③ p62 showed a moderate correlation with IL-10 and IL-1β at the gene level(r=0.57*and-0.69*),and the expression of p62 protein also showed a moderate correlation with IL-6 and IL-10,with correlation coefficients reaching 0.51*and 0.64*,respectively.The expression of LC3 protein showed a good correlation with IL-10 protein expression(r=0.55*,P＜0.05),and ULK1,Atg5,Atg7 genes all showed a good correlation with IL-10(-0.43,-0.67*,-0.62*).关键词
小鼠/肥胖症/运动减脂/自噬活性/炎症反应/作用机制Key words
mice/obesity/exercise-induced fat loss/autophagy activity/inflammatory response/mechanism of action分类
农业科技引用本文复制引用
赵永军,刘绍生,王晶,谢聪聪,何玉秀..运动减脂机制:自噬与炎症的关系及作用[J].扬州大学学报（农业与生命科学版）,2026,47(3):51-60,10.基金项目
山西省哲学社会科学规划项目(2022YJ140) （2022YJ140）
山西大学博士后科研基金资助项目(GJJ2201650) （GJJ2201650）
江西省教育厅科学技术研究一般项目(GJJ220165) （GJJ220165）
井冈山大学博士科研启动(自然科学)项目(JZB2418) （自然科学）
