生物灾害科学2026，Vol.49Issue(2)：205-214,10.DOI:10.3969/j.issn.2095-3704.2026.02.24
马铃薯Y病毒SYRI株系外壳蛋白抗血清制备及应用
Preparation and application of antiserum against the coat protein of potato virus Y SYRI strain
摘要
Abstract
[Objective]Potato virus Y(PVY)is the main pathogen of tobacco and potato virus diseases.SYRI strain is the dominant strain in China.Preparation of coat protein(CP)antiserum of SYRI strain can reduce the missed detection rate of PVY.[Method]The PVY CPSYRI gene sequence was amplified and ligated into the prokaryotic expression vector pEHISTEV to obtain the recombinant plasmid pEHISTEV-CPSYRI.This recombinant plasmid was then transformed into Escherichia coli Rosetta,and after prokaryotic expression,New Zealand white rabbits were immunized to obtain polyclonal antibodies.[Result]Western blot results showed that the prepared antiserum could detect PVY CPSYRI protein diluted 100 000-fold,and it exhibited no cross-reaction with the CP protein of Turnip mosaic virus(TuMV),which belonged to the same genus.Tests on potato and tobacco samples in the field showed that the antiserum prepared was effective in detecting the diseased plants in the field.[Conclusion]The antiserum has showed good sensitivity and specificity,and could be used to detect PVY in crops in the field,which has laid a foundation for the monitoring and early warning of PVY epidemic.关键词
马铃薯Y病毒/SYRI株系/外壳蛋白/抗血清/应用Key words
potato virus Y/SYRI strain/coat protein/antiserum/application分类
农业科技引用本文复制引用
姜松雨,麦钟月,田延平,于伟鹏,何韦韦,李俊,程德杰,李向东..马铃薯Y病毒SYRI株系外壳蛋白抗血清制备及应用[J].生物灾害科学,2026,49(2):205-214,10.基金项目
广西自然科学基金项目(2024GXNSFBA010322、2025GXNSFAA069837) （2024GXNSFBA010322、2025GXNSFAA069837）
