检验医学与临床2026，Vol.23Issue(12)：1605-1610,6.DOI:10.3969/j.issn.1672-9455.2026.12.003
尿液BTA与NMP22联合检测在膀胱癌诊断中的应用
Combined detection of urinary BTA and NMP22 in the diagnosis of bladder cancer
摘要
Abstract
Objective To evaluate the diagnostic value of urinary bladder tumor antigen(BTA)and nuclear matrix protein 22(NMP22)detected by chemiluminescence immunoassay,both alone and in combination,for bladder cancer,and to analyze their correlations with pathological grade.Methods A total of 247 subjects who visited the hospital or underwent physical examination from August 2022 to December 2024 were selected,comprising 80 patients with bladder cancer(bladder cancer group),51 with other urological malignancies(other urological malignancies group),66 with benign urological diseases(benign urological diseases group),and 50 healthy individuals(healthy control group).Based on muscular invasion status,patients with bladder cancer were divided into muscle-invasive bladder cancer(MIBC)and non-muscle-invasive bladder cancer(NMIBC);based on pathological grade,they were divided into G1,G2,and G3 grades.The levels of urinary BTA and NMP22 in all subjects were measured using a fully automated chemiluminescence immunoassay plat-form.The diagnostic performance of urinary BTA and NMP22 for bladder cancer was evaluated using receiver operating characteristic(ROC)curve.Correlations of urinary BTA and NMP22 levels with the pathological grade of bladder cancer were analyzed using Spearman correlation analysis.Results Compared with the other uro-logical malignancies group,the benign urological diseases group and the healthy control group,urinary BTA and NMP22 levels were elevated in the bladder cancer group,and the differences were statistically significant(all P<0.008 3).The areas under the ROC curves(AUCs)of urinary BTA alone,NMP22 alone,and their combination for the diagnosis of bladder cancer were determined to be 0.748,0.768,and 0.780 respectively by ROC curve analysis.Regarding pathological features,significantly elevated BTA and NMP22 levels were measured in the MIBC patients compared with the NMIBC patients(P<0.05).Statistically significant differ-ences in these marker levels were found among patients with different pathological grades(P<0.05);specif-ically,compared with patients with G1 and G2 tumors,significantly higher urinary BTA and NMP22 levels were recorded in patients with G3 tumors(P<0.016 7).Positive correlations of urinary BTA and NMP22 levels with the pathological grade of bladder cancer were demonstrated by Spearman correlation analysis(rs=0.295 and 0.301 respectively;both P<0.01).Conclusion Both urinary BTA and NMP22 have certain diag-nostic value for bladder cancer,and their combined detection improves the overall diagnostic performance.These two markers can serve as reference indicators for the auxiliary diagnosis of bladder cancer.关键词
膀胱癌/膀胱肿瘤抗原/核基质蛋白22/化学发光免疫分析法/诊断Key words
bladder cancer/bladder tumor antigen/nuclear matrix protein 22/chemiluminescence immunoassay/diagnosis分类
医药卫生引用本文复制引用
续晴云,黄海明,李海霞..尿液BTA与NMP22联合检测在膀胱癌诊断中的应用[J].检验医学与临床,2026,23(12):1605-1610,6.基金项目
北京高层次创新创业青年拔尖人才青苗计划项目(G202522094). （G202522094）
