昆明医科大学学报2026，Vol.47Issue(6)：44-55,12.DOI:10.12259/j.issn.2095-610X.S20260605
基于数据挖掘和网络药理学探究中药治疗IgA肾病的核心物质及其分子机制
Core Active Substances and Molecular Mechanisms of Traditional Chinese Medicine in Treating IgA Nephropathy Based on Data Mining and Network Pharmacology
摘要
Abstract
Objective To predict the mechanism of traditional Chinese medicine(TCM)in treating IgA nephropathy(IgAN)based on data mining and network pharmacology,and to verify findings through in vitro experiments.Methods Medical case records for IgAN treatment were retrieved from the Ancient and Modern Medical Case Cloud Platform to screen core drugs.Drug targets were searched in databases including TCMSP,and IgAN targets were searched in databases such as Genecards.Venn diagrams were used to identify intersecting targets,establishing disease-component-target network maps.PPI networks were constructed,followed by GO and KEGG analysis,with molecular docking validation.An IgAN cell model was established with control groups,model groups(IgA),and drug-treated groups at different concentrations(quercetin).Cell viability was detected using CCK-8 assays;IL-6 secretion levels were measured by ELISA;target gene expression levels were detected by RT-qPCR.Results Data mining revealed Astragalus had the highest frequency at 144 occurrences;TCM properties were predominantly cold,sweet,hepatic-targeting,and heat-clearing with detoxifying effects.Network pharmacology analysis identified core substances as Quercetin、Luteolin、Kaempferol、Wogonin and Isorhamnetin.PPI analysis identified core targets as ESR1,IL-6,BCL-2,JUN,and CASP3.Molecular docking verified strong binding affinity between core substances and targets.GO analysis included positive regulation of gene expression;KEGG enrichment pathways included AGE-RAGE signaling and IL-17 signaling pathways.Experimental verification showed that quercetin intervention increased cell viability(P＜0.05);suppressed IL-6 expression(P＜0.01),and upregulated ESR1,BCL-2 mRNA levels(P＜0.05),and downregulating IL-6,JUN,and CASP3 levels(P＜0.05).Conclusion This study identified core therapeutic substances and corresponding targets,and experimentally validated that quercetin enhances cell viability under IgA stimulation,suppresses inflammatory cytokine secretion,and modulates target gene expression,providing valuable insights for investigating molecular mechanisms of IgAN therapy.关键词
IgA肾病/数据挖掘/网络药理学/分子对接/体外实验Key words
IgA nephropathy/Data mining/Network pharmacology/Molecular docking/In vitro experiment分类
医药卫生引用本文复制引用
杨川琪,赵若莲,唐亚娟,刘子杰,李欢,张慧,柴琳..基于数据挖掘和网络药理学探究中药治疗IgA肾病的核心物质及其分子机制[J].昆明医科大学学报,2026,47(6):44-55,12.基金项目
云南省中医药基础研究联合专项基金(202501AZ070001-254) （202501AZ070001-254）
昆明市卫生科研课题(2025-11-01-025) （2025-11-01-025）
昆明市卫生科技人才培养项目医学技术中心卫生科研课题(2023-SW-技术-015) （2023-SW-技术-015）
