兰州大学学报（医学版）2026，Vol.52Issue(4)：19-25,7.DOI:10.13885/j.issn.2097-681X.M20250806
松乳菇多糖上调miR-450b-3p抑制乳腺癌细胞增殖并诱导凋亡
Lactarius deliciosus polysaccharide inhibits the proliferation and induce the apoptosis of human breast cancer cells by upregulating the expression of miR-450b-3p
摘要
Abstract
Objective To explore the effect of Lactarius deliciosus polysaccharide on the proliferation and apoptosis of breast cancer cell MDA-MB-231 and its molecular mechanism.Methods The breast cancer cell line MDA-MB-231 were randomly divided into a control group,low,medium,and high-dose Lactarius deli-ciosus polysaccharide groups,miR-NC group,miR-450b-3p group,and Lactarius deliciosus polysaccharide+anti-miR-NC group,Lactarius deliciosus polysaccharide+anti-miR-450b-3p group;the proliferation inhibition rate was detected by tetramethylazolium salt colorimetric method;clone formation experiment was used to detect cell clone formation ability;flow cytometry was used to detect apoptosis;Western blotting was used to detect protein expression;real-time fluorescent quantitative polymerase chain reaction was preformed to detect the expression of miR-450b-3p.Lactarius deliciosus polysaccharide and miR-450b-3p antagonist or antago-nist NC were used to treat xenografted mice with breast cancer.Tumor volume,mass,expression of miR-450b-3p and number of TUNEL positive cells were measured to confirm the anticancer effect and mechanism of Lactarius deliciosus polysaccharide in vivo.Results The number of clones formed in breast cancer MDA-MB-231 cells treated with low,medium,and high doses of Lactarius deliciosus polysaccharide was increased,and the apoptosis rate,proliferation inhibition rate,the expression levels of miR-450b-3p,cleaved-caspase-3 and cleaved-caspase-9 were increased(P<0.05).After an overexpression of miR-450b-3p,the number of clones formed was decreased,the apoptosis rate,proliferation inhibition rate,and the expression levels of miR-450b-3p,cleaved caspase-3 and cleaved caspase-9 were increased(P<0.05).Inhibition of miR-450b-3p expression weakened the effects of Lactarius deliciosus polysaccharide on the proliferation and apoptosis of breast cancer MDA-MB-231 cells.In vivo,Lactarius deliciosus polysaccharide decreased tumor relative volume and tumor weight,and increased the expression of miR-450b-3p and the rate of TUNEL positive cells(P<0.05).Silenc-ing miR-450b-3p reduced the effects of Lactarius deliciosus polysaccharide on the growth and apoptosis of transplanted tumors.Conclusion Lactarius deliciosus polysaccharide inhibits the proliferation of breast can-cer MDA-MB-231 and induces its apoptosis by up-regulating the expression of miR-450b-3p.关键词
松乳菇多糖/乳腺癌/增殖/凋亡/线粒体凋亡通路/异种移植瘤Key words
Lactarius deliciosus polysaccharide/breast cancer/proliferation/apoptosis/mitochondrial apop-totic pathway/xenotransplanted tumor分类
医药卫生引用本文复制引用
赵翊,刘宇杰,张高飞..松乳菇多糖上调miR-450b-3p抑制乳腺癌细胞增殖并诱导凋亡[J].兰州大学学报（医学版）,2026,52(4):19-25,7.基金项目
陕西省重点研发计划资助项目(2023-YBSF-329) （2023-YBSF-329）
陕西省人民医院科技发展孵化基金资助项目(2023YJY-49) （2023YJY-49）
