临床输血与检验2026，Vol.28Issue(3)：332-339,8.DOI:10.3969/j.issn.1671-2587.2026.03.006
沉默MIB2抑制甲状腺乳头状癌细胞的实验研究
MIB2 Knockdown Exerts Tumor-suppressive Effects in Papillary Thyroid Carcinoma Cells
摘要
Abstract
Objective Bioinformatic analyses have identified aberrant overexpression of E3 ubiquitin ligase Mindbomb 2(MIB2)in papillary thyroid carcinoma(PTC),implicating it as a key driver of PTC tumorigenesis.This study characterizes the functional impacts of MIB2 silencing on proliferation,apoptosis,invasion,and migration in the PTC-derived TPC-1 cell line.Methods MIB2-silenced TPC-1 cell lines were generated via RNA interference(RNAi).Cells were divided into control(TPC-1)and MIB2-silenced(TPC-1-MIB2 RNAi)groups.Cell proliferative capacity was quantified using Cell Counting Kit-8(CCK-8)and colony formation assays.Invasive and migratory potentials were evaluated via Transwell invasion and wound healing assays,respectively.Flow cytometry was performed to assess cell cycle distribution and apoptotic rates.Results Compared with control cells,TPC-1-MIB2 RNAi group exhibited significantly attenuated proliferation kinetics and reduced colony formation efficiency,consistent with robust suppression of PTC cell proliferation following MIB2 knockdown(P＜0.05).Flow cytometric profiling revealed prominent G0/G1 phase cell cycle arrest in TPC-1-MIB2 RNAi group,coupled with a marked elevation in apoptotic rate compared with control cells,indicative of enhanced PTC cell apoptosis mediated by MIB2 silencing(P＜0.05).Transwell invasion and wound healing assays demonstrated that TPC-1-MIB2 RNAi cells markedly impaired the invasive and migratory capacities of TPC-1 cells,as evidenced by reduced transmembrane invasion and significantly delayed wound closure compared to TPC-1 group(P＜0.05).Conclusion MIB2 silencing exerts potent tumor-suppressive effects in PTC cells through inhibition of proliferation,invasion,and migration,alongside concomitant promotion of apoptosis.These findings identify MIB2 as a promising candidate therapeutic target for papillary thyroid carcinoma.关键词
甲状腺乳头状细胞/MIB2/增殖/凋亡/侵袭与迁移Key words
Papillary thyroid carcinoma/Mindbomb 2(MIB2)/Cell proliferation/Apoptosis/Tumor invasion and migration分类
医药卫生引用本文复制引用
樊瑞军,王羽佳,谢文华,贾咏存,郭临池..沉默MIB2抑制甲状腺乳头状癌细胞的实验研究[J].临床输血与检验,2026,28(3):332-339,8.基金项目
本课题受宁夏自然科学基金(No.2023AAC03455) （No.2023AAC03455）
2023年肿瘤微环境与免疫治疗湖北省重点实验室(三峡大学)开放基金(No.2023KZL033) （三峡大学）
宜昌市医疗卫生研究项目(No.A24-2-045) （No.A24-2-045）
湖北省自然科学基金(No.2024AFC040)资助 （No.2024AFC040）
