临床输血与检验2026，Vol.28Issue(3)：359-363,5.DOI:10.3969/j.issn.1671-2587.2026.03.010
冻干富血小板血浆对体外人真皮成纤维细胞增殖和迁移的影响
Effect of Freeze-dried Platelet-rich Plasma on the Proliferation and Migration of Human Dermal Fibroblasts in Vitro
李艳辉 1皮丽萍 1谢婷婷 1周谋 1施琳颖1
作者信息
- 1. 中国人民解放军南部战区总医院,广东 广州 510010
- 折叠
摘要
Abstract
Objective To evaluate the capacity of freeze-dried platelet-rich plasma(FD-PRP)to stimulate the proliferation and migration of human dermal fibroblasts.Methods Freshly isolated PRP was lyophilized to generate FD-PRP.Cell proliferation was quantified using the CCK-8 assay;directional migration was monitored via scratch-wound assays;and the secretion of type Ⅰ collagen and elastin was measured by ELISA.Results Compared to untreated controls,FD-PRP significantly enhanced fibroblast proliferation and increased the secretion of type Ⅰ collagen and elastin(P＜0.05).These effects were statistically comparable to those induced by fresh PRP(P＞0.05).Scratch-wound assays further revealed that both FD-PRP and fresh PRP markedly enhanced cell migration into the denuded area compared with the controls.Conclusion FD-PRP induces the proliferation and migration of adult dermal fibroblasts and also enhances the secretion of type Ⅰ collagen and elastin.These findings lay a solid experimental foundation for the potential clinical application of FD-PRP in the future treatment of various wound types.关键词
富血小板血浆/冻干/人成纤维细胞/创面Key words
Platelet rich plasma/Freeze-dried/Human fibroblasts/Wound分类
医药卫生引用本文复制引用
李艳辉,皮丽萍,谢婷婷,周谋,施琳颖..冻干富血小板血浆对体外人真皮成纤维细胞增殖和迁移的影响[J].临床输血与检验,2026,28(3):359-363,5.
