麦类作物学报2026，Vol.46Issue(6)：754-767,14.DOI:10.7606/j.issn.1009-1041.2026.06.06
基于SSR分子标记的青稞品种鉴定及遗传多样性分析
SSR Marker Based Analysis for Identification and Genetic Diversity of Hulless Barley Varieties
摘要
Abstract
To establish a molecular identification system and DNA fingerprint database for hulless bar-ley(Hordeum vulgare L.var.nudum Hook.f.)varieties in China,this study aimed to screen a set of SSR core primers suitable for national variety identification standards,thereby providing technical support for variety authentication,management,and new variety protection.Based on previously re-ported SSR markers of hulless barley,PCR amplification,marker screening,and validation were per-formed across diverse varieties,followed by analyses of genetic diversity and discrimination efficiency.Using 155 hulless barley accessions,200 SSR primer pairs were subjected to polymorphism screening.Twenty-one core primers were selected based on criteria including clear and easily interpretable peak patterns,high polymorphism(mean PIC=0.68),amplification stability,and even chromosomal dis-tribution.Reference varieties(e.g.,Yunqing 4,Diqing 4,and Kunlun 8)were employed to validate the stability and reliability of these primers.The constructed DNA fingerprint database for 155 acces-sions detected 137 alleles,ranging from 2 to 10 per locus(mean=6.52).The polymorphism informa-tion content(PIC)values varied between 0.39 and 0.85(mean=0.68),with 95.24％of loci classified as highly polymorphic(PIC＞0.5).These 21 primers achieved a discrimination efficiency of 99.991％,distinguishing 153 out of 155 accessions.Cluster analysis,population structure analysis,and principal component analysis classified the 154 accessions into three distinct groups,revealing rich genetic di-versity and simple genetic backgrounds in 45.81％of the accessions.The SSR-based identification system developed in this study demonstrated high accuracy and practical applicability,enabling au-thenticity verification,auxiliary screening of approximate varieties in DUS testing,and intellectual property protection.Furthermore,the DNA fingerprint database and genetic diversity analysis pro-vide valuable molecular-level insights for future hulless barley breeding programs.关键词
青稞/SSR标记/品种鉴定/遗传多样性Key words
Hulless barley/SSR markers/Variety identification/Genetic diversity分类
农业科技引用本文复制引用
滕彩玲,杨晓洪,荆若男,张凯淅,法永英,赵春芳,韩瑞玺,刘艳芳..基于SSR分子标记的青稞品种鉴定及遗传多样性分析[J].麦类作物学报,2026,46(6):754-767,14.基金项目
科技部生物育成品种DUS测试工具装备研发项目(2023ZD0406106) （2023ZD0406106）
云南省重大科技专项(202502AE090022) （202502AE090022）
云南省重点研发计划项目(202503AP140031) （202503AP140031）
云南省农业科学院预研项目 （）
中国科学技术部国际杰青计划项目(国科外[2025]28号) （国科外[2025]28号）
