国际眼科杂志2026，Vol.26Issue(7)：1239-1245,7.DOI:10.3980/j.issn.1672-5123.2026.7.22
年龄相关性白内障患者血清Sirt1-6水平与氧化应激指标的关系及其诊断价值
Relationship between serum Sirt1-6 levels and oxidative stress indicators in age-related cataract patients and their diagnostic value
摘要
Abstract
·AIM:To investigate the relationship between serum levels of sirtuins(Sirts)1-6 and oxidative stress markers,and to evaluate their diagnostic value for age-related cataract(ARC). ·METHODS:A prospective cohort of ARC patients admitted to the hospital between June 2019 and July 2021 was enrolled as the study subjects.Concurrently,age-matched healthy individuals undergoing routine physical examinations at the hospital during the same period were recruited as the control group.Baseline demographic and clinical characteristics were compared between the two groups.Serum levels of Sirt1-6 and oxidative stress markers including malondialdehyde(MDA),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),and total antioxidant capacity(TAC)were compared.Pearson correlation analysis was performed to assess the associations between serum Sirt1-6 levels and oxidative stress parameters.Multivariate Logistic regression analysis was conducted to analysis risk factors associated with ARC occurrence.Receiver operating characteristic(ROC)curve was used to evaluate the diagnostic value of serum Sirt1-6 levels and oxidative stress indicators for ARC. ·RESULTS;The study included 127 ARC patients,comprising 68 men and 59 women,with a mean age of 66.05±2.58 y;the concurrent control group consisted of 121 patients,comprising 63 men and 58 women,with a mean age of 65.54±2.86 y.Serum levels of Sirt1,3 and 6,SOD,GSH-Px,and TAC were significantly lower in the ARC group compared to the control group(all P＜0.001),whereas MDA levels were markedly elevated(P＜0.001).Pearson correlation analysis revealed that serum levels of Sirt1,Sirt3,Sirt6 in the ARC group were positively correlated with SOD,GSH-Px,and TAC,and negatively correlated with MDA.Multivariate Logistic regression analysis demonstrated that serum Sirt1,Sirt6,MDA,SOD,and GSH-Px were significantly associated with the occurrence of ARC(all P＜0.001).ROC curve analysis showed that the combination of Sirt1,Sirt6,MDA,SOD,and GSH-Px yielded an area under the curve(AUC)of 0.995 for diagnosing ARC,which was significantly higher than that of Sirt1 alone(Z=4.978,P＜0.001),Sirt6 alone(Z=7.487,P＜0.001),MDA alone(Z=6.449,P＜0.001),SOD alone(Z=5.773,P＜0.001),or GSH-Px alone(Z=5.056,P＜0.001),indicating superior diagnostic accuracy of the multimarker panel(P＜0.05). ·CONCLUSION;Serum levels of Sirt1,3,and 6 are generally reduced in ARC patients and are closely associated with oxidative stress imbalance.Specifically,decreased serum levels of Sirt1 and Sirt6,along with oxidative stress markers(elevated MDA and reduced SOD and GSH-Px)are identified as risk factors for ARC.Moreover,the combined detection of these indicators presents high diagnostic value for ARC.关键词
沉默信息调节因子(Sirts)/年龄相关性白内障/氧化应激/诊断价值Key words
silent information regulator(Sirts)/age-related cataract/oxidative stress/diagnostic value引用本文复制引用
路露,吴芳芳,吴文文,杨立东,刘雅雯..年龄相关性白内障患者血清Sirt1-6水平与氧化应激指标的关系及其诊断价值[J].国际眼科杂志,2026,26(7):1239-1245,7.基金项目
沧州市重点研发计划指导项目(No.213106143) Key Research and Development Project of Cangzhou City(No.213106143) （No.213106143）
