内蒙古民族大学学报（自然科学版）2026，Vol.41Issue(3)：49-58,10.DOI:10.14045/j.cnki.15-1220.2026.03.007
RcGSTF11作为潜在光合调节物的双效调节机制研究
Study on the Dual Regulatory Mechanism of RcGSTF11 Acting as a Potential Photosynthetic Regulator
摘要
Abstract
To investigate the mechanism through which GSTF acts as a potential photosynthetic regulator in photo-synthesis,this study overexpressed the castor bean RcGSTF11 gene in Arabidopsis thaliana.It was found that the plant height and silique yield of RcGSTF11-OE Arabidopsis plants were significantly higher than those of the wild type,and the net photosynthetic rate(Pn)increased by approximately 28.31%.The interaction between Rc GSTF11 and the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco),Rc RBCL,was verified by surface plasmon reso-nance(SPR)and bimolecular fluorescence complementation(BiFC)assays.The Rubisco activity in RcGSTF11-OE Ara-bidopsis plants significantly increased.Combined analysis of transcriptomics and metabolomics enriched the differen-tially expressed genes in photosynthetic antenna proteins,photosynthesis,and plant hormone signal transduction path-ways.It was relatively found that sugar substances accumulated in RcGSTF11-OE Arabidopsis plants.In addition,the expression of RcGSTF11 can reduce the accumulation of ROS,increase the GSH/GSSG ratio,and help maintain the re-dox homeostasis in plants and reshape the transcription of downstream genes.In summary,Rc GSTF11 was found to have a dual regulatory effect of proximal interaction with RBCL to regulate photosynthetic carbon assimilation and distal regulation of redox homeostasis.关键词
RcGSTF11/核酮糖-1,5-二磷酸羧化/加氧酶/光合作用/抗氧化/RBCLKey words
RcGSTF11/ribulose-1,5-bisphosphate carboxylase/oxygenase/photosynthesis/antioxidation/RBCL分类
农业科技引用本文复制引用
赵艺,李哲,张春玲,刘涵淼,任宪锋,李国瑞..RcGSTF11作为潜在光合调节物的双效调节机制研究[J].内蒙古民族大学学报（自然科学版）,2026,41(3):49-58,10.基金项目
内蒙古自治区蓖麻育种重点实验室建设项目(2025KYPT0089) （2025KYPT0089）
内蒙古自治区自然科学基金项目(2022LHQN03010) （2022LHQN03010）
