青岛大学学报（医学版）2026，Vol.62Issue(2)：292-296,5.DOI:10.11712/jms.2096-5532.2026.62.056
EBV相关胃癌细胞中LMP2A对KLF5的表达调控作用
Regulatory effect of latent membrane protein 2A on Krüppel-like factor 5 in Epstein-Barr virus-associated gastric carcinoma cells
摘要
Abstract
Objective To investigate the change in the expression of Krüppel-like factor 5(KLF5)in Epstein-Barr virus-as-sociated gastric carcinoma(EBVaGC)tissue and cells,as well as the regulatory effect of EBV-encoded latent membrane protein 2A(LMP2A)on KLF5 and the effect of KLF5 on the expression of the EBV lytic gene BZLF1 in EBVaGC cells.Methods Immu-nohistochemistry and Western blot were used to measure the expression of KLF5 in EBVaGC/EBV-negative gastric carcinoma(EBVnGC)tissue and cell lines.SGC7901 and SNU719 cells were transfected with LMP2A plasmid and siRNA,and the expression of KLF5 was observed.KLF5 was overexpressed in SNU719 cells,and the expression of BZLF1 was measured.Results The ex-pression level of KLF5 in EBVaGC tissue and cell lines was significantly lower than that in EBVnGC tissue and cell lines(t=6.125,P＜0.001).After LMP2A was overexpressed in SGC7901 cells,the expression of KLF5 was significantly downregulated(t=10.650,P＜0.001),and after LMP2A was knocked down in SNU719 cells,the expression of KLF5 was significantly upregulated(t=4.615,P＜0.01).Overexpression of KLF5 in SNU719 cells significantly upregulated the expression of BZLF1(t=5.741,P＜0.01).Conclusion EBV can inhibit the expression of KLF5 in EBVaGC tissue and cells.In EBVaGC cells,LMP2A can down-regulate the expression of KLF5,while KLF5 can upregulate the expression of the EBV lytic gene BZLF1.关键词
疱疹病毒4型,人/胃肿瘤/Krüppel样因子5/潜伏膜蛋白2A/BZLF1Key words
herpesvirus 4,human/stomach neoplasms/Krüppel-like factor 5/latent membrane protein 2A/BZLF1分类
医药卫生引用本文复制引用
卢青晴,刘丹丹,师朵,许琳,刘术臻..EBV相关胃癌细胞中LMP2A对KLF5的表达调控作用[J].青岛大学学报（医学版）,2026,62(2):292-296,5.基金项目
青岛西海岸新区科技惠民项目(2019-61) （2019-61）
青岛大学附属医院优秀青年基金(QDFY202017) （QDFY202017）
