热带农业工程2026，Vol.50Issue(3)：89-93,5.
Rsc基因转化高效诱导玉米花青素合成及籽粒富集的研究
Study on High-efficiency Induction of Maize Anthocyanin Synthesis and Grain Enrichment Through Rsc Gene Transformation
摘要
Abstract
Anthocyanins,as an important class of plant secondary metabolites,hold broad application prospects in the fields of food and medicine.Maize(Zea mays L.),as a major food crop,requires the study of its anthocyanin biosynthetic mechanisms for quality improvement.This study focused on the key transcription factor gene Rsc(Zm00001eb089420_T001)in the anthocyanin synthesis path-way.Using Agrobacterium-mediated transformation,the maize embryogenic callus was genetically transformed with an expression vector,yielding four purple-phenotype grains.PCR and Southern hy-bridization assays revealed that 11.11％of the T1 generation population exhibited constitutive purple coloration throughout the plant,with deep purple grains in self-pollinated ears,indicating that strong Rsc gene expression stably drives anthocyanin accumulation in all plant tissues and grains.Anthocyanin composition analysis showed that the total anthocyanin content in grains was the highest(427.68±1.90 mg/100g DW),significantly higher than in stems(372.08±8.44 mg/100g DW).Among the three major monomers,luteolin had the highest content(296.78±0.49 mg/100g DW in male flow-ers);apigenin accumulated significantly higher in reproductive organs(grains,male flowers)than in vegetative organs(roots,stems,leaves);and peony pigment was highest in roots(49.25±6.09 mg/100g DW).This study confirmed that genetic transformation of Rsc gene could efficiently induce anthocyanin synthesis in multiple tissues of maize.As the key organ for anthocyanin accumulation,maize grain provided a material basis for creating high anthocyanin maize germplasm.关键词
花青素/玉米/遗传转化/种质Key words
anthocyanin/maize/genetic transformation/germplasm分类
农业科技引用本文复制引用
李梦婷,张艳鑫,陈智慧,张佳,黄兴,杜邓襄..Rsc基因转化高效诱导玉米花青素合成及籽粒富集的研究[J].热带农业工程,2026,50(3):89-93,5.基金项目
武汉轻工大学引进(培养)人才科研启动资金. （培养）
