热带农业科学2026，Vol.46Issue(5)：52-56,5.DOI:10.12008/j.issn.1009-2196.2026.05.007
珍稀植物蒜头果外植体消毒及诱导启动培养研究
Explant Disinfection and Induction Initiation Culture of the Rare Plants Malania oleifera
摘要
Abstract
Taking the terminal buds and axillary buds of M.oleifera as experimental materials,aseptic disinfection technol-ogy and induction initiation culture technology for M.oleifera explants were studied.The results showed that the optimal dis-infection method for M.oleifera explants was as follows:disinfected with 75%alcohol for one min and rinsed twice with ster-ile water.the samples were centrifuged at a speed of 3 000～4 000 r·min-1 for five min,disinfected in NaClO for 5 min,and rinsed 5 times with sterile water.Then the samples were placed in a glass bottle containing 0.1%HgCl2,disinfected for 10 min,and finally rinsed five times with sterile water.The explants treated by this disinfection method had a low contamination rate,which was conducive to explant transfer and initiation culture.The most suitable medium for M.oleifera was induction initia-tion culture with modified MS+0.2 mg·L-1 6-BA+0.2 mg·L-1 NAA.The plants induced by this medium grew the fastest,had the most branches.关键词
蒜头果/外植体/消毒/启动培养Key words
Malania oleifera/explant/disinfection/initiation culture分类
农业科技引用本文复制引用
卢娟,张烨,余春和,岑祚舟,孙熹,李莉莉..珍稀植物蒜头果外植体消毒及诱导启动培养研究[J].热带农业科学,2026,46(5):52-56,5.基金项目
广西林业科技推广示范项目(No.2024GXLK28) （No.2024GXLK28）
广西自筹经费林业科技项目(No.2023GXZCLK34) （No.2023GXZCLK34）
玉市科攻20173026. （）
