热带农业科学2026，Vol.46Issue(5)：97-101,5.DOI:10.12008/j.issn.1009-2196.2026.05.013
榴莲花粉活力测定方法的比较研究
Comparative Study on the Determination Methods of Pollen Viability of Durio zibethinus
摘要
Abstract
In this study,two durian cultivars,'MonThong'(Jinzhen)and'Musang King'(Maoshanwang),were used as ex-perimental materials.Pollens at the full bloom stage were collected,and the pollen viability of durian stored for 2,4,8,12,18,and 24 hours was determined by the Alexander staining method and MTT staining method.In addition,the pollen germination rates at different culture times were observed via ex-vitro germination method(solid and liquid ex vitro germination methods).The results showed that the pollen viability of both cultivars showed a decreasing trend with the extension of storage time,with no significant difference within 2-4 hours;the pollen viability of'MonThong'(Jinzhen)was higher than that of'Musang King'(Maoshanwang)from 8 to 12 hours,and the pollen viability was basically lost after 24 hours,indicating that pollen storage under normal temperature and dry conditions is only suitable for short-term storage within 8 hours.The Alexander staining method is more suitable for the rapid detection of durian pollen viability and its level,while the ex-vitro germination method,although time-consuming,has more accurate and reliable results;the combination of the two methods can improve the detection accuracy.This study provides a scientific basis for optimizing the timing of artificial durian pollination,formulating pollen preservation strategies,and promoting hybrid breeding.关键词
榴莲/花粉活力/亚历山大染色法/MTT染色法/离体培养萌发法Key words
Durio zibethinus/pollen viability/alexander staining methods/MTT staining method/in vitro germination method分类
农业科技引用本文复制引用
王清琳,邵本浩,周兆禧,何书强,朱润华,林兴娥..榴莲花粉活力测定方法的比较研究[J].热带农业科学,2026,46(5):97-101,5.基金项目
海南省重点研发项目(No.ZDYF2024XDNY174、No.ZDYF2023XDNY178) （No.ZDYF2024XDNY174、No.ZDYF2023XDNY178）
中央级基本科研业务费(No.JSJCSF-2025005). （No.JSJCSF-2025005）
