四川农业大学学报2026，Vol.44Issue(3)：589-597,607,10.DOI:10.16036/j.issn.1000-2650.202603739
根用芥菜小孢子培养技术体系的建立及抗根肿病DH种质的创制
Establishment of A Microspore Culture System and Development of Clubroot-Resistant DH Germplasm in Brassica juncea var.napiformis
摘要
Abstract
[Objective]This study aimed to establish an efficient microspore culture system for tuberous mustard(Brassica juncea var.napiformis)and to create breeding materials resistant to clubroot disease.[Method]Key steps including microspore developmental stage,embryoid induction,plant regenera-tion,and ploidy identification were optimized,and the regenerated DH(doubled haploid)populations were evaluated for disease resistance.[Result]The optimal microspore developmental stage for culture corresponded to bud lengths of 2.0-4.0 mm,at which the microspores were at the late uninucleate stage.Following induction with heat shock at 33 ℃ for 2 days,plant regeneration was achieved on the opti-mized medium.Flow cytometry analysis revealed a spontaneous diploidization rate of 9.57％,and three resistant lines were screened from 26 DH lines.[Conclusion]A complete technical procedure from mi-crospore culture to disease resistance evaluation was established for tuberous mustard,confirming its practical value in the rapid development of homozygous breeding materials.关键词
根用芥菜/小孢子培养/双单倍体/根肿病/抗性育种Key words
Brassica juncea var.napiformis/microspore culture/doubled haploid/clubroot/resistance breeding分类
农业科技引用本文复制引用
喻小娇,杨霄,周延,金鑫,张英,贺兵,何翠,张晓禹,罗玉华,王杰,李元会,齐放..根用芥菜小孢子培养技术体系的建立及抗根肿病DH种质的创制[J].四川农业大学学报,2026,44(3):589-597,607,10.基金项目
宜宾市农业科技创新(2024NYCX017). （2024NYCX017）
