四川农业大学学报2026，Vol.44Issue(3)：598-607,10.DOI:10.16036/j.issn.1000-2650.202512733
甘蓝型油菜ABI3基因的克隆、表达分析和功能的初步研究
Cloning,Expression Analysis,and Preliminary Functional Research of the ABI3 Gene in Brassica napus
摘要
Abstract
[Objective]To clone the coding sequence(CDS)of the Brassica napus ABI3 gene,analyze the structural features of the BnABI3 gene,the properties of its encoded protein,and its expression pat-tern under abiotic stress,thereby providing insights into the regulatory role of the ABI3 gene in respond-ing to abiotic stress.[Method]Using leaf cDNA from Brassica napus"Zhongyou 821"as a template,the BnABI3 gene CDS sequence was cloned.Bioinformatics analysis was performed,and its expression under abiotic stress was analyzed using qRT-PCR.Protein interactions were verified through Y2H and BiFC ex-periments.[Result]The CDS region of BnABI3 gene in B.napus was 2 133 bp in length,encoding 710 amino acids.The homology with AtABI3 protein sequence in A.thaliana was 84.33%.The BnABI3 gene responds to drought,low temperature,and salt stress treatments,exhibiting sharply induced expression under salt stress with a response intensity higher than other stress treatments.The BnABI3 protein local-ized to the nucleus,and yeast two-hybrid and bimolecular fluorescence complementation analyses indi-cated its interaction with the transcription factor BnABI5.[Conclusion]BnABI3 is a nuclear-localized protein that likely mediates BnABI5-regulated biological activities through interaction with BnABI5,par-ticipating in Brassica napus responses to drought,low temperature,and salt stress.These findings pro-vide insights into the regulatory mechanisms of BnABI3 stress resistance and lay the foundation for further functional studies of the BnABI3 gene.关键词
甘蓝型油菜/BnABI3/非生物胁迫/表达分析Key words
Brassica napus/BnABI3/abiotic stress/expression analysis分类
农业科技引用本文复制引用
唐永康,唐海娟,黄磊,赵玲,牛文靖,郑颖,李壮..甘蓝型油菜ABI3基因的克隆、表达分析和功能的初步研究[J].四川农业大学学报,2026,44(3):598-607,10.基金项目
西南作物基因资源发掘与利用国家重点实验室自由探索(SKL-ZY202218). （SKL-ZY202218）
