水产学报2026，Vol.50Issue(6)：59-67,9.DOI:10.11964/jfc.20260315461
黑海参断裂再生过程内部形态变化及对溶解态外源营养物的吸收
Morphological changes of internal organs and absorption of exogenous dissolved nutrients during fission and regeneration in the sea cucumber(Holothuria atra)
摘要
Abstract
This study investigated the morphological changes of internal organs in the black sea cucumber Holothuria atra dur-ing fission and regeneration,as well as its absorption and utilization of exogenous dissolved nutrients.Fission was artificially induced by rubber band ligation,and the internal organ changes in the anterior and posterior segments were observed through dissection at 0,10,30,50,and 60 days(d)post-fission.Based on stable isotope tracing,labeled dissolved amino acids(δ13C,δ15N)and glucose(δ18O)were added to the water at 10,30,and 60 d of regeneration,respectively.Intact H.atra without the addition of exogenous nutrients served as the control group.The changes in isotope ratios in the body wall tissues of the intact group,anterior parts,and posterior parts were compared.Morphological observations showed that at 60 days post-fission,the anterior parts completely regenerated the intestine,respiratory trees,and cloaca,while the posterior parts reconstructed the intestine,pharyngeal complex,and tentacles.Both parts successfully restored a complete digestive system.Isotope tracing res-ults indicated that the addition of exogenous dissolved nutrients significantly altered the isotopic composition of the regenerat-ing body wall tissues.Compared with the control group,the δ15N values of both anterior and posterior individuals in the experi-mental group increased significantly at all regeneration stages,indicating effective absorption of amino acids during regenera-tion.The δ13C values in the posterior parts were significantly higher than those in the control group at 30 days.Regarding gluc-ose absorption,the δ18O values of the anterior tissues were significantly higher than those of the posterior tissues after adding exogenous nutrients,suggesting potential differences in absorption efficiency or metabolic pathways between the different body sections.The study demonstrates that H.atra requires 60 days to complete the full remodeling of the digestive system and res-piratory trees following fission.Both dissolved amino acids and glucose can be absorbed during regeneration,with the anterior segments exhibiting a higher glucose uptake than the posterior segments.This study confirms that dissolved organic matter(DOM)is a crucial exogenous nutritional supplement during the regeneration of H.atra,revealing the differential utilization of exogenous nutrients across various regeneration stages and body parts.These findings provide a theoretical basis for elucidat-ing the energy allocation strategies and nutritional compensation mechanisms in echinoderm regeneration.关键词
黑海参/断裂再生/稳定同位素示踪/溶解态有机物/营养吸收Key words
Holothuria atra/fission and regeneration/stable isotope tracing/dissolved organic matter/nutrient absorption分类
农业科技引用本文复制引用
谢静婷,赵燕,王海青..黑海参断裂再生过程内部形态变化及对溶解态外源营养物的吸收[J].水产学报,2026,50(6):59-67,9.基金项目
国家自然科学基金(42106113) （42106113）
国家重点研发计划(2024YFD2401802) （2024YFD2401802）
海南省研究生教育创新计划(Hys2025-125) National Natural Science Foundation of China(42106113) （Hys2025-125）
National Key Research and Development Pro-gram of China(2024YFD2401802) （2024YFD2401802）
Hainan Provincial Postgraduate Education Innovation Project(Hys2025-125) （Hys2025-125）
