山东农业科学2026，Vol.58Issue(5)：40-49,10.DOI:10.14083/j.issn.1001-4942.2026.05.005
北苍术全长转录组测序及分子标记开发
Full-Length Transcriptome Sequencing and Molecular Marker Development of Atractylodes chinensis(DC.)Koidz
摘要
Abstract
To construct a full-length transcriptome database of Atractylodes chinensis(DC.)Koidz and mine molecular markers,transcriptome sequencing was performed using single molecule real-time(SMRT)technology with roots,stems and leaves as experimental materials.The key enzyme genes potentially involved in terpenoid biosynthesis were identified,and the applicability of SSR molecular markers in A.chinensis and A.lancea was preliminarily evaluated.The results showed that after de-redundancy analysis,a total of 14 556 high-quality full-length transcripts were obtained,of which,13 962(95.92％)were successfully annotated in five public databases(KEGG,GO,Nr,Uniprot and Pfam).KEGG annotation analysis indicated that 11 907 transcripts were assigned to 126 pathways with the largest number of transcripts annotated to metabolism-relat-ed pathways as 6 606.A total of 133 transcripts involved in terpenoid and polyketide metabolisms were identi-fied,and 12 transcripts were mapped to the sesquiterpenoid and triterpenoid biosynthesis pathways;59 lncR-NAs and 14 438 CDS sequences were obtained,4 443 SSR loci and 688 transcription factors were detected,and the GRAS transcription factor family owned the most members.Some randomly selected SSR molecular markers were successfully amplified in A.chinensis and A.lancea.These findings could provide new insights into the molecular mechanisms underlying sesquiterpene biosynthesis in Atractylodes,as well as provide bases for molecular marker development and mining and utilization of functional genes.关键词
北苍术/全长转录组/功能注释/倍半萜/SSR分子标记Key words
Atractylodes chinensis(DC.)Koidz/Full-length transcriptome/Function annotation/Ses-quiterpenes/SSR molecular markers分类
农业科技引用本文复制引用
王晖,邓娟,张改霞,胡楠,高妍夏,黄露,张争,王彬彬..北苍术全长转录组测序及分子标记开发[J].山东农业科学,2026,58(5):40-49,10.基金项目
国家重点研发计划项目(2022YFC3501502) （2022YFC3501502）
河北省自然科学基金项目(H2022406031) （H2022406031）
河北省中医药管理局科研计划项目(2024118) （2024118）
承德医学院基本科研业务费资助项目(KY202405) （KY202405）
