世界科学技术-中医药现代化2026，Vol.28Issue(6)：1868-1882,15.DOI:10.11842/wst.20250608002
基于UHPLC-Q-TOF-MS/MS技术揭示丹参水提液在急性血瘀证大鼠中的成分动态分布特征
Elucidating Dynamic Distribution of Components in Salvia miltiorrhiza Extract in Rats with Acute Blood Stasis Syndrome by UHPLC-Q-TOF-MS/MS
摘要
Abstract
Objective To systematically investigate the dynamic distribution of Salvia miltiorrhiza(SM)extract in acute blood stasis model rats by UHPLC-Q-TOF-MS/MS,and explore its multi-component and multi-organ synergistic characteristics,thereby providing a scientific data for the pharmacodynamic material foundation elucidation of its blood-activating and stasis-resolving effects.Methods Fifteen Sprague-Dawley rats were randomly divided into blank group,model group and SM extract group.Rats in the model group and SM extract group were induced as acute blood stasis syndrome,and rats in the SM extract group were given SM extract at a dose of 5.4 g·kg-1 for 10 consecutive days.Biological samples from the SM extract group were analyzed by UHPLC-Q-TOF-MS/MS in both positive and negative ionization modes.Plasma was collected at different timepoints including 0.25,0.5,1,2,and 4 h on day 11.Organs likes heart,liver,spleen,lung,kidney,and colon,and excreta like urine and feces were also collected on day 11.The contents of prototype components were determined and compared among groups using peak area-based relative quantification method.Results A total of 133 compounds were identified in the SM extract.40 prototype components were detected in plasma at different timepoints.Tissue distribution characteristic was observed as colon(54)>lung(48)>liver(21)>kidney(19)>spleen(11)>heart(10).In addition,48 and 65 prototypes were detected in urine and feces,respectively.Diterpenoids such as tanshinone Ⅱ A,and phenolic acids such as salvianolic acid B were the predominant constituents in the blood stasis model rats,which plasma concentration peaked at 1 h post administration.The prototype exposure varied significantly among the detected organs:colon>lung>kidney>liver>heart>spleen.Notably,stachyose was found in the plasma and the colon tissue for the first time.The prototype compounds showed higher quantities and contents in the feces than those in the urine.Conclusion This study systematically firstly reveals the in vivo distribution profile of SM extract in a pathological model,and establish a visual spectrum of"component-multi-tissue response".The dynamic distribution patterns of the compounds emphasized the important role of stachyose in multi-system interactions to remodel organismal homeostasis when Salvia miltiorrhiza treating acute blood stasis syndrome,as well as the diterpenoids and phenolic acids.关键词
丹参水提液/超高效液相色谱-四极杆-飞行时间质谱联用技术/急性血瘀证/成分动态分布Key words
Salvia Miltiorrhiza extract/UHPLC-Q-TOF-MS/MS/Acute blood stasis syndrome/Dynamic component distribution分类
医药卫生引用本文复制引用
杨丽媛,唐婷婷,王超,李娟,高洁,庞涛,张凤,陈万生..基于UHPLC-Q-TOF-MS/MS技术揭示丹参水提液在急性血瘀证大鼠中的成分动态分布特征[J].世界科学技术-中医药现代化,2026,28(6):1868-1882,15.基金项目
科技部重点研发计划(2022YFC3501700):临床证据导向的丹参、党参"功效-物质"全景解析及深度研发,负责人:陈万生 （2022YFC3501700）
上海市科学技术委员会项目(22S21901900):治疗心律失常制剂益心饮的临床前研究,负责人:张凤 （22S21901900）
上海中医药大学有组织科研重点专项(2023YZZ02):基于物质-功效解析的气血理论科学阐释,负责人:陈万生. （2023YZZ02）
