世界中医药2026，Vol.21Issue(6)：966-975,10.DOI:10.3969/j.issn.1673-7202.2026.06.002
基于网络药理学和实验验证黄芪赤风汤治疗脑梗死的作用机制
Mechanisms of Huangqi Chifeng Decoction in Treating Cerebral Infarction Based on Network Pharmacology and Experimental Verification
摘要
Abstract
Objective:To investigate the molecular mechanisms underlying the therapeutic effects of Huangqi Chifeng Decoction on cerebral infarction using network pharmacology and animal experiments.Methods:Network pharmacology was employed to identify the active components of Huangqi Chifeng Decoction and their potential targets associated with cerebral infarction.Molecular doc-king was performed to verify the interactions between the major active compounds and core targets,and the findings were further validated through animal experiments.Results:A total of 56 active compounds and 298 targets were identified.Molecular docking a-nalysis demonstrated that five key compounds could directly interact with seven core targets,and the core compounds exhibited fa-vorable binding affinities with the core proteins.Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis indicated that Huangqi Chifeng Decoction may exert therapeutic effects on cerebral infarction primarily through the phosphoinositide 3-kinase/protein kinase B(PI3K/AKT1)signaling pathway,tumor necrosis factor(TNF)signaling pathway,interleukin-17(IL-17)signaling pathway,lipid and atherosclerosis pathway,advanced glycation end product-receptor for advanced glycation end product(AGE-RAGE)signaling pathway,and fluid shear stress and atherosclerosis pathway.Experimental validation demonstrated that Huangqi Chifeng Decoction improved neurological deficits in rats,reduced the levels of IL-6,TNF,and IL-1β in peripheral serum,and improved the expression of AKT1 and TNF in brain tissue.Conclusion:The therapeutic effects of Huangqi Chifeng Decoction on cerebral infarction may be associated with the regulation of core targets,including AKT1,TNF,IL-6,and IL-1β.关键词
脑梗死/黄芪赤风汤/网络药理学/分子对接/实验研究/作用机制/信号通路/有效成分Key words
Cerebral infarction/Huangqi Chifeng Decoction/Network pharmacology/Molecular docking/Experimental research/Mechanism of action/Signaling pathway/Active ingredient分类
医药卫生引用本文复制引用
原筝,秦合伟,邢若星..基于网络药理学和实验验证黄芪赤风汤治疗脑梗死的作用机制[J].世界中医药,2026,21(6):966-975,10.基金项目
国家自然科学基金面上项目(82374551) （82374551）
河南省科技攻关项目(252102311268) （252102311268）
河南省卫生健康中青年学科带头人项目(豫人卫[2024]3号) （豫人卫[2024]3号）
河南中医药大学"仲景三名人才工程"仲景青年名家(20250917) （20250917）
河南省中医药科学研究专项普通课题(2025ZY3061,2025ZY3062). （2025ZY3061,2025ZY3062）
