世界中医药2026，Vol.21Issue(6)：1016-1021,1029,7.DOI:10.3969/j.issn.1673-7202.2026.06.008
骨乐冲剂抑制成骨细胞程序性坏死机制研究
Mechanisms of Gule Infusion Granules in Suppression of Necroptosis of Osteoblasts
摘要
Abstract
Objective:To investigate the mechanisms by which Gule Infusion Granules inhibit necroptosis in osteoblasts.Methods:Osteoblasts were isolated from the calvaria of neonatal rats,and a cellular model of osteoblast necroptosis was established by treat-ment with resibufogenin(RBG).The cells were divided into a control group without intervention,a model group treated with 20 μmol/L RBG,and a Gule Infusion Granules group treated with 20 μmol/L RBG in combination with high-dose Gule Infusion Granules-containing serum.Cell viability,alkaline phosphatase(ALP)activity,intracellular ultrastructural changes,mitochondrial permeability transition pore(MPTP)opening,reactive oxygen species(ROS)levels,and the expression of proteins associated with the RIPK1/RIPK3/MLKL signaling pathway were evaluated and compared among the groups.Results:Compared with the control group,osteoblasts in the RBG model group exhibited significantly reduced cell viability and ALP activity.In contrast,ROS levels,MPTP opening,and the expression levels of the key necroptosis-related proteins RIPK1,RIPK3,and MLKL were significantly in-creased.Treatment with Gule Infusion Granules significantly reversed these changes.Transmission electron microscopy further dem-onstrated that cells in the model group displayed typical ultrastructural features of necroptosis,whereas these morphological altera-tions were markedly improved in the Gule Infusion Granules group.Conclusion:Gule Infusion Granules inhibits osteoblast necrop-tosis and promotes osteoblast differentiation by activating the RIPK1/RIPK3/MLKL signaling pathway.关键词
骨乐冲剂/受体相互作用丝氨酸/苏氨酸蛋白激酶1/受体相互作用丝氨酸/苏氨酸蛋白激酶3/混合谱系激酶结构域样蛋白/成骨细胞/增殖/分化/氧化应激/线粒体膜通透性/程序性坏死Key words
Gule Infusion Granules/RIPK1/RIPK3/MLKL/Osteoblasts/Proliferation/Differentiation/Oxidative stress/Mito-chondrial membrane permeability/Necroptosis分类
医药卫生引用本文复制引用
孙贵才,徐轶尔,刘阳,杨文钊,卢美峰,万亭,郑贵浩..骨乐冲剂抑制成骨细胞程序性坏死机制研究[J].世界中医药,2026,21(6):1016-1021,1029,7.基金项目
国家自然科学基金项目(82260870). （82260870）
