世界中医药2026，Vol.21Issue(6)：1022-1029,8.DOI:10.3969/j.issn.1673-7202.2026.06.009
加味桂枝茯苓汤通过线粒体自噬通路对高糖雪旺细胞损伤的影响
Effects of Jiawei Guizhi Fuling Decoction against High Glucose-induced Schwann Cell Damage through Mitophagy Pathway
摘要
Abstract
Objective:To investigate the protective mechanisms of Jiawei Guizhi Fuling Decoction(JGFD)against high glucose-in-duced injury in rat Schwann cells(RSC96)through the PTEN-induced putative kinase 1/Parkin(PINK1/Parkin)signaling path-way.Methods:RSC96 cell injury was induced by high glucose,and 50 mmol/L glucose was selected as the optimal concentration.Cell Counting Kit-8(CCK-8)assay identified 15％JGFD-containing serum as the optimal concentration.Cells were divided into the normal group,model group(50 mmol/L glucose+15％blank serum),JGFD group(50 mmol/L glucose+15％JGFD-containing serum),and siRNA-PINK1 group(siRNA-PINK1 transfection).PINK1 transfection efficiency was verified by Western blot.Mito-chondrial morphology and autophagic structures were observed by transmission electron microscopy.The protein and mRNA expres-sion levels of PINK1,Parkin,P62,Beclin-1,and microtubule-associated protein 1 light chain 3(LC3)were determined by Western blot and real-time quantitative PCR(RT-qPCR),respectively.Reactive oxygen species(ROS)levels were measured by flow cytom-etry.Results:High glucose significantly injured RSC96 cells,and 50 mmol/L glucose resulted in relatively higher cell viability.The protective effect of 15％JGFD-containing serum was optimal.Compared with the normal group,ROS levels were significantly in-creased in the model group(P＜0.01),whereas the expression levels of PINK1,Parkin,Beclin-1,and LC3 were significantly de-creased and P62 expression was significantly increased(P＜0.01).Severe cellular damage and the absence of autophagic structures were also observed.Compared with the model group,the JGFD group exhibited significantly reduced ROS levels(P＜0.01),rever-sal of the above molecular expression changes(P＜0.05 or P＜0.01),alleviated cellular damage,and restoration of autophagic structures.In contrast,the siRNA-PINK1 group reversed the protective effects of JGFD(P＜0.01),accompanied by aggravated cel-lular damage and loss of autophagic structures.Conclusion:JGFD exerts a protective effect against RSC96 cell injury by alleviating cellular damage through PINK1/Parkin signaling pathway-mediated mitophagy.关键词
加味桂枝茯苓汤/痛性糖尿病周围神经病变/雪旺细胞/含药血清/线粒体自噬/PTEN诱导假定激酶1/帕金森病蛋白信号通路/活性氧/氧化应激Key words
Jiawei Guizhi Fuling Decoction/Diabetic peripheral neuropathy/Schwann cells/Drug-containing serum/Mitophagy/PTEN-induced putative kinase 1/Parkinson's disease protein signaling pathway/Reactive oxygen species/Oxidative stress分类
医药卫生引用本文复制引用
刘爱华,冷锦红,孙新雨,沈歆媛,黄一铭,林庶茹..加味桂枝茯苓汤通过线粒体自噬通路对高糖雪旺细胞损伤的影响[J].世界中医药,2026,21(6):1022-1029,8.基金项目
国家自然科学基金面上项目(81072912)——益气活血法对痛性糖尿病周围神经病变离子通道功能障碍影响的机制研究 （81072912）
张静生国医大师传承工作室项目(2023-1) （2023-1）
辽宁省教育厅2022年度高等学校基本科研项目(LJKMZ20221332) （LJKMZ20221332）
沈阳市科技计划项目(22-321-33-39). （22-321-33-39）
