水生态学杂志2026，Vol.47Issue(4)：219-231,13.DOI:10.15928/j.1674-3075.202404220148
患传染性脾肾坏死病翘嘴鳜养殖池塘水质及水体微生物群落结构分析
Water Quality and Microbial Community Structure in Aquaculture Ponds of Mandarin Fish(Siniperca chuatsi)Infected by the Spleen and Kidney Necrosis Virus
摘要
Abstract
Microorganisms in the aquatic environment are crucial to aquatic ecosystems,playing impor-tant roles in pond water quality,disease prevention,nutrient dynamics and energy cycling.In this study,we analyzed physicochemical factors and microbial community structure of the water body in mandarin fish(Siniperca chuatsi)aquaculture ponds under healthy conditions and during outbreaks of infectious spleen and kidney necrosis virus(ISKNV).Our aim was to understand the dynamic changes in water qual-ity and bacterial community structure following an ISKNV outbreak From July to September of 2022,wa-ter samples were collected from two aquaculture ponds with healthy or infected fish.Water quality indica-tors for the two ponds before and after the ISKNV outbreak were determined and microbial community structure was determined using 16S rDNA sequencing technology.Results showed increased accumula-tion rates of alkalinity,ammonia nitrogen,nitrate nitrogen,total nitrogen,and total phosphorus in pond water after the ISKNV outbreak.The ISKNV outbreak decreased the self-purification capacity of the pond and changed the microbial community structure.The dominant bacterial phyla in the healthy S.ch-uatsi pond were Proteobacteria,Bacteroidetes,and Firmicutes.The ISKNV outbreak decreased microbial richness and diversity in the pond,decreased the relative abundance of Proteobacteria and increased the relative abundance of Bacteroidetes(an indicator of pollution)and significantly increased the relative abundances of the genera Thermomonas,Gemmobacter,and Legionella.Pearson correlation analysis indi-cated that genera such as Candidatus Aquiluna,the CL500-29 marine group,hgcl clade,Stenotrophomon-as,Cyanobium PCC-6307,and unclassified Illumatobacteraceae could potentially act as nitrogen-removal probiotics in the mandarin fish culture ponds and play a role in regulating water quality in aquaculture.关键词
水体理化因子/传染性脾肾坏死病毒/翘嘴鳜/高通量测序/微生物群落结构Key words
water physiochemical parameters/infectious spleen and kidney necrosis virus(ISKNV)/Siniperca chuatsi/high-throughput sequencing/microbial community structure分类
农业科技引用本文复制引用
蒋利进,王淼,姜志勇,吴郁丽,曹建萌,衣萌萌,可小丽,刘志刚,谭泽加,卢迈新..患传染性脾肾坏死病翘嘴鳜养殖池塘水质及水体微生物群落结构分析[J].水生态学杂志,2026,47(4):219-231,13.基金项目
财政部和农业农村部资助项目(CARS-46) （CARS-46）
粤财农[2022]119号资助项目 （）
广州市科技计划资助项目(2023E04J0002,2024B03J1264). （2023E04J0002,2024B03J1264）
