中国生态农业学报（中英文）2026，Vol.34Issue(6)：1196-1207,12.DOI:10.12357/cjea.20250151
东北黑土区土地利用方式对土壤酶活性及其化学计量比的影响
Effects of different land use types on soil enzyme activities and their stoichiometric ratios in the black soil region of Northeast China
摘要
Abstract
Soil enzyme activity is a fundamental biochemical indicator that reflects microbial metabolic functioning and the intensity of nutrient cycling processes within soil ecosystems.Understanding how soil enzyme activity,enzymatic stoichiometry,and microbial nutrient limitation respond to land use change is essential for developing effective soil management strategies,particularly in the black soil region of Northeast China,where maintaining soil fertility and ecological stability is of critical importance.In this study,a total of 78 soil samples were collected from representative farmland and forest sites through a systematic grid sampling design to ensure comprehensive spatial coverage.The activities of β-1,4-glucosidase(BG),β-N-acetylglucosaminidase(NAG),leucine aminopeptidase(LAP),and acid phosphatase(AP)were quantified,and their stoichiometric characteristics were analyzed to assess microbial nutrient-acquisition strategies.Redundancy analysis was further conducted to explore the interactive relationships among enzyme activities,microbial nutrient limitation,soil physicochemical properties,and climatic variables.The results showed that forest soils exhibited increases of 74.35％(P＜0.05),108.70％(P＜0.01),80.12％(P＜0.05),and 31.87％(P＜0.05)in activities of BG,NAG,LAP,and AP,respectively,compared with farmland soils,which indicated that forest ecosystems generally supported more active microbial metabolism and stronger nutrient cycling potential.Redundancy analysis demonstrated that soil pH was the most important factor explaining the spatial variations in enzyme activities,with the explanatory power of R2=0.74.Mean annual precipitation and ammonium-nitrogen content were also influential,accounting for 31％(R2=0.31)and 30％(R2=0.30)of the total variation,respectively.The enzymatic stoichiometric ratio associated with microbial acquisition of carbon,nitrogen,and phosphorus was 1∶1.34∶1.58,indicating disproportionate microbial investment toward nitrogen-and especially phosphorus-acquiring enzymes.Both farmland and forest soils exhibited clear microbial phosphorus limitation,while the limitation was more pronounced in farmland soils.This stronger phosphorus limitation in farmland was likely resulted from long-term tillage intensity and unbalanced fertilization practices,which caused relative enrichment of carbon and nitrogen,altered microbial community structure,reduced soil phosphorus availability,and constrained microbial functional potential.Soil pH,mean annual precipitation,and ammonium-nitrogen content were identified as the key environmental drivers regulating soil enzyme activity on the regional scale.Overall,the findings suggest that ecologically informed soil management strategies,including rational phosphorus fertilization,optimized water management,and reduced soil disturbance,are essential for alleviating microbial phosphorus limitation,enhancing soil nutrient cycling efficiency,and promoting long-term ecological sustainability in the black soil region of Northeast China.关键词
土地利用方式/土壤酶活性/化学计量比/pH/养分限制Key words
land use type/soil enzyme activity/stoichiometric ratio/pH/nutrient limitation分类
农业科技引用本文复制引用
黄炎炎,朱恒侠,王钒旭,吴勇,曾兵,吴会军,宋霄君..东北黑土区土地利用方式对土壤酶活性及其化学计量比的影响[J].中国生态农业学报（中英文）,2026,34(6):1196-1207,12.基金项目
国家重点研发计划(2021YFD1500201)和中国农业科学院科技创新工程重大科研任务(CAAS-ZDRW202202)资助 This study was supported by the National Key Research and Development Program of China(2021YFD1500201)and the Major Research Task of Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences(CAAS-ZDRW202202). （2021YFD1500201）
