时珍国医国药2026，Vol.37Issue(12)：2212-2220,9.DOI:10.70976/j.1008-0805.SZGYGY-2026-1202
大泻脾汤通过靶向FOXK1-糖酵解轴逆转胃癌代谢重编程与诱导凋亡的研究
Mechanistic study of Daxiepi Decoction(大泻脾汤)in reversing metabolic reprogramming and inducing apoptosis in gastric cancer by targeting the FOXK1-glycolysis axis
摘要
Abstract
Objective To investigate the mechanism by which Daxiepi Decoction(大泻脾汤,DXP)reverses metabolic reprogramming and induces apoptosis in gastric cancer cells through regulating the Forkhead box K1(FOXK1)-mediated glycolytic pathway.Methods An FOXK1-overexpressing gastric cancer HGC-27 cell model was established.Cells were treated with drug-containing serum.The effects of DXP were assessed using the CCK-8 assay,EdU staining,immunofluorescence(IF),qPCR,and Western blot(WB).Mea-surements included HK2,PKM2,LDHA,glucose metabolism parameters(glucose consumption,lactate production,adenosine triphos-phate/ATP levels),and apoptosis-related markers(Bcl-2,Bcl-2-associated X protein/Bax,and caspase-3).Results FOXK1 overex-pression promoted gastric cancer cell proliferation,up-regulated mRNA expression of GLUT1,HK2,PKM2,and LDHA,and sup-pressed apoptosis,indicated by increased Bcl-2 and decreased Bax and caspase-3 mRNA levels.Treatment with 30％-40％DXP-containing serum for 72 h significantly inhibited FOXK1 expression(P<0.01),increased glucose content,and decreased lactate pro-duction and ATP levels.Additionally,Bcl-2 expression was reduced,while Bax and caspase-3 were elevated.Combination experi-ments showed that DXP antagonized the metabolic reprogramming and anti-apoptotic effects induced by FOXK1 overexpression.Conclu-sion DXP may suppress malignant proliferation of gastric cancer cells by targeting the FOXK1-glycolysis axis and reversing the Warburg effect-driven metabolic-apoptotic imbalance.This study provides preliminary molecular evidence for the theory of"simultaneously regu-lating the structure and function"in Dunhuang medicine and suggests a potential new strategy for Chinese herbal medicine(CHM)com-pounds in targeting tumor metabolic reprogramming.关键词
大泻脾汤/胃癌/叉头框转录因子K1/糖酵解/细胞凋亡/代谢重编程Key words
Daxiepi Decoction(大泻脾汤)/Gastric cancer/FOXK1/Glycolysis/Apoptosis/Metabolic reprogramming分类
医药卫生引用本文复制引用
郭昊铭,李武龙,汪学鹏,卓淇泓,候晓琳,杨永琴,魏本君..大泻脾汤通过靶向FOXK1-糖酵解轴逆转胃癌代谢重编程与诱导凋亡的研究[J].时珍国医国药,2026,37(12):2212-2220,9.基金项目
国家自然科学基金(82260893) （82260893）
甘肃省自然科学基金资助项目(24JRRA1023) （24JRRA1023）
敦煌医学与转化教育部重点实验室开放基金(DHYX2023-03) （DHYX2023-03）
