时珍国医国药2026，Vol.37Issue(12)：2221-2227,7.DOI:10.70976/j.1008-0805.SZGYGY-2026-1203
基于"态靶"思维的丹参红花配伍靶向P-Smad3与脑缺血再灌注损伤的相关性研究
Study on the correlation between targeted P-Smad3 and cerebral ischemia-reperfusion injury under the state-target concept for the Radix Salviae Miltiorrhizae and Flos Carthami medici-nal couplet
摘要
Abstract
Objective To investigate the protective effects of the Radix Salviae Miltiorrhizae and Flos Carthami medicinal couplet on cerebral ischemia-reperfusion injury(CIRI)and its association with P-Smad3.Methods An in vitro oxygen-glucose deprivation/reoxy-genation(OGD/R)model was established in hCMEC/d3 cells,which were divided into Control,OGD/R,DH,SIS3 HCl,and SIS3 HCl+DH groups.Cell viability,lactate dehydrogenase(LDH)release,P-Smad3 expression,and migration ability were assessed.In vivo,a middle cerebral artery occlusion(MCAO)model was induced in SD rats,which were randomized into Sham,MCAO,DH,SIS3 HCl,SIS3 HCl+DH,and Edaravone groups.Brain tissues were examined by hematoxylin-eosin staining(HE),immunohistochemistry(IHC),and Western blot(WB).Results Optimal modeling conditions were OGD for 6 h and drug concentrations of 85-680 µmol/L.Compared with the OGD/R group,the DH group showed significantly increased cell viability and migration,reduced LDH release,and elevated P-Smad3 expression,whereas SIS3 HCl exerted the opposite effects.The combination of SIS3 HCl with DH reversed the effects of SIS3 HCl alone.Consistent results were observed in vivo:MCAO rats exhibited neuronal loss,nuclear pyknosis,and elevated P-Smad3,which were alleviated by DH and Edaravone with further enhancement of P-Smad3.SIS3 HCl aggravated pathological damage and suppressed P-Smad3,while its combination with DH partially restored protective effects.Conclusion The Radix Salviae Miltiorrhi-zae and Flos Carthami medicinal couplet attenuates CIRI-induced cellular injury and enhances cell migration,potentially through nega-tive regulation of the P-Smad3 signaling pathway.关键词
态靶/脑缺血再灌注损伤/丹参红花/P-Smad3/磷酸化Key words
State-target/Cerebral ischemia-reperfusion injury/Radix Salviae Miltiorrhizae and Flos Carthami medicinal cou-plet/P-Smad3/Phosphorylation分类
医药卫生引用本文复制引用
朱潇,朱佳馨,徐青云,李文武,李刚,雷烨,张怡敏,朱慧渊,苗琦..基于"态靶"思维的丹参红花配伍靶向P-Smad3与脑缺血再灌注损伤的相关性研究[J].时珍国医国药,2026,37(12):2221-2227,7.基金项目
国家自然科学基金(82174268) （82174268）
国家科技重大专项(2024ZD0523504) （2024ZD0523504）
