时珍国医国药2026，Vol.37Issue(12)：2241-2247,7.DOI:10.70976/j.1008-0805.SZGYGY-2026-1206
基于氧化应激及PI3K/AKT通路的补气活血益精方改善肾虚血瘀型少弱精子症大鼠睾丸组织的研究
Investigating the mechanism of Buqi Huoxue Yijing Formula(补气活血益精方)on testicular tissue in oligoasthenospermia rats with kidney deficiency and blood stasis type based on oxida-tive stress and the PI3K/AKT pathway
摘要
Abstract
Objective To investigate the ameliorative effect of Buqi Huoxue Yijing Formula(补气活血益精方,BHYF)on oxidative stress damage in the testes of rats with kidney deficiency and blood stasis-type oligoasthenospermia(OAS)and its regulatory mechanism on the phosphoinositide 3-kinase(PI3K)/protein kinase B(AKT)signaling pathway.Methods Sixty male SD rats were randomly divided into a blank group(n=10)and a modeling group(n=50).The model was established by intragastric administration of Tripter-ygium glycosides(40 mg·kg-1·d-1).After successful modeling,the rats were further divided into the model group,L-carnitine group(1.8 mL·kg-1·d-1),and low/medium/high-dose BHYF groups(7.87,15.74,and 31.5 g·kg-1·d-1,respectively).Treatments contin-ued for 28 days.At the end of the 8-week experimental period,bilateral testicular tissues were harvested.The following indicators were assessed:the general condition of rats;sperm quality using an automated sperm analyzer;histopathological changes in testicular tissue by hematoxylin-eosin(HE)staining;levels of reactive oxygen species(ROS)and malondialdehyde(MDA)in testicular tissue by enzyme-linked immunosorbent assay(ELISA);and protein expression of PI3K,p-PI3K,AKT,and p-AKT by Western blot(WB).Results Compared with the blank group,the model group exhibited significantly reduced sperm concentration and motility,structural disruption of seminiferous tubules,markedly elevated levels of ROS and MDA(P<0.01),and significantly decreased expression of PI3K,p-PI3K,AKT,and p-AKT proteins(P<0.01).All treatment groups(especially the high-dose group)effectively restored semi-niferous tubule structure and significantly reduced ROS and MDA levels(P<0.01,P<0.05).Except for the low-dose group,all other treatment groups significantly upregulated the expression of PI3K/AKT pathway-related proteins(P<0.01).Conclusion BHYF may improve testicular spermatogenic function by regulating oxidative stress and activating the PI3K/AKT signaling pathway.关键词
补气活血益精方/肾虚血瘀型少弱精子症/PI3K/AKT信号通路/睾丸组织/氧化应激Key words
Buqi Huoxue Yijing Formula(补气活血益精方)/Oligoasthenospermia of kidney deficiency and blood stasis type/PI3K/AKT signaling pathway/Testicular tissue/Oxidative stress分类
医药卫生引用本文复制引用
常家庆,邢喜平,鞠海超,赵威威,马丽丽,赵娟,李启明,王雪阳,杨佳树,王新平..基于氧化应激及PI3K/AKT通路的补气活血益精方改善肾虚血瘀型少弱精子症大鼠睾丸组织的研究[J].时珍国医国药,2026,37(12):2241-2247,7.基金项目
国家自然科学基金(82560880) （82560880）
甘肃省卫生健康行业科技创新重大科研项目(GSWSZD2024-18) （GSWSZD2024-18）
甘肃省中医药管理局项目(GZKP-2023-15) （GZKP-2023-15）
甘肃省自然科学基金项目(25JRRA969) （25JRRA969）
甘肃省卫生健康行业骨干人才项目(甘卫人函[2023]398号) （甘卫人函[2023]398号）
