时珍国医国药2026，Vol.37Issue(12)：2248-2256,9.DOI:10.70976/j.1008-0805.SZGYGY-2026-1207
参芪地黄汤化裁方含药血清对高糖诱导的小鼠永生化足细胞焦亡的保护作用影响
The protective effect of the serum containing the modified prescription of Shenqi Dihuang Decoction(参芪地黄汤)on high-glucose-induced pyroptosis in MPC5 cells
摘要
Abstract
Objective To investigate the protective effect of serum containing Shenqi Dihuang Decoction modified prescription(参芪地黄汤,SQDH)against pyroptosis in immortalized mouse podocytes induced by high glucose.Methods Mouse podocytes(MPC5)were cultured in vitro.Optimal intervention concentrations of high glucose and SQDH-containing serum,as well as exposure duration,were determined.Cells were divided into four groups:normal control,high glucose model,SQDH-containing serum,and NLRP3 inhibitor control(MCC950 group).Morphological changes indicative of pyroptosis were observed under light microscopy.Levels of IL-1β and IL-18 in cell supernatants were measured by enzyme-linked immunosorbent assay(ELISA).Protein expression of Nephrin,Podocin,PODXL,WT-1,Desmin,NLRP3,Cleaved Caspase-1,and GSDMD-N was detected via Western blot(WB).Immunofluorescence was used to assess expression levels of Nephrin,NLRP3,GSDMD-N,and Cleaved Caspase-1.Results Compared with the normal control group,the high glucose model group exhibited disrupted cell membrane integrity and reduced cell density.ELISA results showed upregulated expression of IL-1β and IL-18(P<0.05).Western blot analysis revealed decreased expression of Nephrin,Podocin,PODXL,and WT-1(P<0.05),and increased expression of Desmin,NLRP3,Cleaved Caspase-1,and GSDMD-N(P<0.05).Immu-nofluorescence indicated elevated relative fluorescence intensity of NLRP3,Cleaved Caspase-1,and GSDMD-N(P<0.05),alongside reduced Nephrin expression(P<0.05).Both the SQDH-containing serum group and MCC950 group showed significant amelioration of these abnormalities compared to the model group(P<0.05).Conclusion Serum containing SQDH modified prescription alleviates high glucose-induced podocyte injury,potentially by mitigating pyroptosis.关键词
足细胞/细胞焦亡/参芪地黄汤/高糖Key words
Podocyte/Pyroptosis/Shenqi Dihuang Decoction(参芪地黄汤)/High glucose分类
医药卫生引用本文复制引用
黄硕,吴睿,杨宇峰,杨新莉,刘松岩,王诗怡,张晖,孙贵炎,石岩..参芪地黄汤化裁方含药血清对高糖诱导的小鼠永生化足细胞焦亡的保护作用影响[J].时珍国医国药,2026,37(12):2248-2256,9.基金项目
国家中医药管理局青年岐黄学者项目(2022年) （2022年）
辽宁省中央引导地方科技发展专项(2019JH6/10200009) （2019JH6/10200009）
辽宁省"兴辽英才计划"青年拔尖人才项目(XLYC1807145) （XLYC1807145）
辽宁省科技计划联合计划自然科学基金-博士科研启动项目(2024-BSLH-152) （2024-BSLH-152）
