时珍国医国药2026，Vol.37Issue(12)：2271-2278,8.DOI:10.70976/j.1008-0805.SZGYGY-2026-1210
基于TLC-HPLC的多基原臭茉莉质量控制方法建立与评价
Establishment and evaluation of a quality control method for multi-origin Clerodendrum phillppinum Schauer var.simplex based on TLC-HPLC
摘要
Abstract
Objective Clerodendrum phillppinum Schauer var.simplex(Clerodendrum)is a genuine medicinal material in Lingnan,and its quality standard is imperfect.In this paper,the quality control method of Clerodendrum is systematically studied to provide a basis for the formulation of its quality standard.Methods A total of 30 batches of Clerodendrum medicinal materials from Lingnan area were collected,contents of water,total ash,and water-soluble extract of Clerodendrum were determined according to the 2025 edition of Chinese Pharmacopoeia(中国药典).To establish a thin layer chromatography(TLC)identification method for Clerodendrum,based on high-performance liquid chromatography(HPLC),the fingerprint and the content determination method of verbascoside were estab-lished.Results The average contents of water,total ash content and water-soluble extract content of 30 batches of Clerodendrum were 4.60％,3.25％and 15.02％,respectively.The established TLC method was good,and the results showed that multiple batches of Clerodendrum showed the same color spots at the same Rf.The established HPLC method was stable in the linear range of 0.004-0.101 mg·mL-1(r=1.000).The content of verbascoside in 30 batches of Clerodendrum was 0.021-0.738％.The fingerprints of multiple batches of Clerodendrum were similar.5 common peaks were identified in the fingerprints,and the 2 common peaks were identified as verbascoside and isoverbascoside.Conclusion The quality control methods such as content determination and fingerprint of Cleroden-drum were established,which provided the key experimental basis for the improvement of the quality standard of Clerodendrum.关键词
臭茉莉/薄层色谱鉴别/指纹图谱/质量标准Key words
Clerodendrum phillppinum Schauer var.simplex/TLC identification method/Fingerprint/Quality standard分类
医药卫生引用本文复制引用
夏体雨,乔卫林,果德安,安亚玲,刘小康,冯琳,蒋慧玲,李振伟,张待娣,赖敏玲,彭丽华..基于TLC-HPLC的多基原臭茉莉质量控制方法建立与评价[J].时珍国医国药,2026,37(12):2271-2278,8.基金项目
广东省重点领域研发计划(2020B1111110007) （2020B1111110007）
