时珍国医国药2026，Vol.37Issue(13)：2401-2409,9.DOI:10.70976/j.1008-0805.SZGYGY-2026-1301
加味玉屏风散调控TNF-α/NF-κB通路改善肥胖型哮喘作用机制的网络药理学及实验验证研究
Exploring the therapeutic effect of Jiawei Yupingfeng Powder(加味玉屏风散)on obese asthma through the TNF-α/NF-κB pathway based on network pharmacology and experimental vali-dation
摘要
Abstract
Objective To elucidate the potential mechanism of Jiawei Yupingfeng Powder(加味玉屏风散,JYP)in treating obese asthma by integrating network pharmacology and animal experiments.Methods The active components of JYP and potential targets for obese asthma were collected and analyzed using TCMSP,GeneCards,and STRING databases to explore the mechanism of action.An obese asthma mouse model was established by high-fat diet(HFD)feeding and ovalbumin(OVA)sensitization/challenge.The mice were treated with JYP or the positive control drug dexamethasone(DEX).The proportion of inflammatory cells in bronchoalveolar lavage fluid(BALF)was detected by flow cytometry(FCM).Hematoxylin-eosin(HE)staining was used to observe inflammatory infiltration in lung tissue and hepatic steatosis.Periodic acid-Schiff(PAS)staining was performed to evaluate peribronchial mucus secretion.Body weight and serum levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),triglycerides(TG),cholesterol(TC),and lactate dehydrogenase(LDH)were measured.The mRNA expression of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),and IL-6 in lung tissue was detected by quantitative polymerase chain reaction(qPCR).Protein expression of TNF-α,NF-κB,and phosphorylated NF-κB(p-NF-κB)in lung tissue was analyzed by Western blot(WB).Results Network pharmacology analysis sug-gested that the TNF signaling pathway might be involved in the therapeutic effect of JYP on obese asthma.Experimental results showed that,compared with the control group,obese asthmatic mice exhibited significantly increased inflammatory cells in BALF;lung histopa-thology revealed inflammatory cell infiltration,airway narrowing,and bronchial epithelial hyperplasia;liver histopathology showed lipid deposition;serum liver function markers and lipid levels were elevated;and mRNA expression of IL-6,IL-1β,and TNF-α in lung tissue was significantly upregulated.JYP treatment reversed these alterations and suppressed the protein expression related to the TNF-α/NF-κB pathway.Conclusion The integrated approach of network pharmacology and experimental validation suggests that JYP may ameliorate obese asthma by mitigating inflammatory injury mediated through the TNF-α/NF-κB pathway.关键词
肥胖型哮喘/网络药理学/加味玉屏风散/中医药/炎症Key words
Obese asthma/Network pharmacology/Jiawei Yupingfeng Powder(加味玉屏风散)/TCM/Inflammation分类
医药卫生引用本文复制引用
蒋雨薇,张惠勇,陈林锦,王钰,马子风,李翠,崔洁,鹿振辉..加味玉屏风散调控TNF-α/NF-κB通路改善肥胖型哮喘作用机制的网络药理学及实验验证研究[J].时珍国医国药,2026,37(13):2401-2409,9.基金项目
国家自然科学基金青年项目(82205004) （82205004）
国家自然科学基金面上项目(82374349,82274263) （82374349,82274263）
上海市卫生健康委员会中医药创新团队(2022CX010) （2022CX010）
中国中医药多学科创新团队(ZYYCXTD--202208) （ZYYCXTD--202208）
上海市加强公共卫生体系建设三年行动计划2023--2025(GWVI-11.1-08) （GWVI-11.1-08）
