时珍国医国药2026，Vol.37Issue(13)：2456-2463,8.DOI:10.70976/j.1008-0805.SZGYGY-2026-1308
清半夏对OVA小鼠的抗炎祛痰作用及机制研究
Study on the anti-inflammatory and expectorant effects and mechanism of Pinelliae Rhizoma Praeparatum Cum Alumine on OVA mice
摘要
Abstract
Objective To investigate the anti-inflammatory and expectorant effects and mechanism of the water extract of Pinelliae Rhi-zoma Praeparatum Cum Alumine(PRAWE),a processed product of Rhizoma Pinelliae,in ovalbumin(OVA)-induced asthmatic mice.Methods HE staining,Masson staining,and AB-PAS staining were used to observe the pathological changes in mouse lung tissues.Untargeted metabolomics technology was applied to analyze the metabolic regulation of PRAWE in OVA-induced mice.ELISA kits were used to detect the levels of immunoglobulin E(IgE)in serum,and tumor necrosis factor-α(TNF-α),interleukin(IL)-6,and IL-1β in bronchoalveolar lavage fluid.Western blot(WB)was performed to detect the expression of proteins related to the nuclear factor kappa B(NF-κB)/NOD-like receptor protein 3(NLRP3)signaling pathway in mouse lung tissues.Results Compared with the OVA group,PRAWE treatment at various doses alleviated inflammatory infiltration,reduced collagen fiber deposition,and decreased mucus secre-tion in the lung tissues of mice.PRAWE regulated metabolic disorders in the lung tissues of OVA-induced mice by affecting pathways such as nucleotide metabolism and pyrimidine metabolism.ELISA results showed that compared with the OVA group,PRAWE down-regulated the levels of IgE in serum and TNF-α,IL-6,and IL-1β in bronchoalveolar lavage fluid(P<0.01 or P<0.05).WB results showed that PRAWE significantly inhibited the expression of phosphorylated(p)-p65,p-inhibitor of kappa B alpha(IκBα),NLRP3,apoptosis-associated speck-like protein containing a CARD(ASC),and caspase-1(P<0.05 or P<0.01).Conclusion PRAWE can effectively alleviate inflammation,reduce mucus secretion,and regulate metabolic disorders in OVA-induced mice.It exerts anti-inflammatory and expectorant effects in OVA-induced asthmatic mice by influencing the NF-κB/NLRP3 signaling pathway.关键词
清半夏/哮喘/非靶向代谢组学/NF-κB/NLRP3信号通路/抗炎祛痰Key words
Pinelliae Rhizoma Praeparatum Cum Alumine/Asthma/Untargeted metabolomics/NF-κB/NLRP3 signaling path-way/Anti-inflammatory and expectorant分类
医药卫生引用本文复制引用
徐承,张淑淇,何欣美,尤朋涛..清半夏对OVA小鼠的抗炎祛痰作用及机制研究[J].时珍国医国药,2026,37(13):2456-2463,8.基金项目
湖北省自然科学基金(2024AFD255) （2024AFD255）
