时珍国医国药2026，Vol.37Issue(13)：2464-2470,7.DOI:10.70976/j.1008-0805.SZGYGY-2026-1309
基于UPLC-Q Exactive Plus Orbitrap MS和UPLC-MS的益脑膏化学成分定性和定量研究
Qualitative and quantitative study of chemical constituents of Yinao Ointment(益脑膏)based on UPLC-Q Exactive Plus Orbitrap MS and UPLC-MS
摘要
Abstract
Objective To establish qualitative and quantitative analysis methods for the chemical constituents of Yinao Ointment(益脑膏,YNO)and clarify its material basis.Methods Chromatographic separation was performed on a Thermo Fisher Scientific Accucore Phenyl Hexyl column(2.1 mm×100 mm,2.6 μm)with gradient elution using 0.1％formic acid aqueous solution-acetonitrile as the mobile phase at a flow rate of 0.2 mL/min.The column temperature was maintained at 35 ℃.Using a heated electrospray ionization source,chemical constituents in YNO were identified through Full Scan/ddMS² mode,combined with comparisons of retention times of reference substances,mass spectrometry ion information,and relevant mass spectrometry data from databases and literature.Further-more,an UPLC-MS method was developed for the simultaneous determination of 24 components in YNO using multiple reaction monitor-ing(MRM)mode scanning.Results Through comparison with reference substances and high-resolution mass spectrometry analysis,116 components were identified and their fragmentation patterns were elucidated.On this basis,an UPLC-MS quantitative method was established for the simultaneous determination of 24 components in YNO.These 24 components showed good linear relationships within their respective detection concentration ranges(r>0.9992).The RSDs for precision,repeatability,and stability were all less than 6.46％,and the average recoveries ranged from 96％to 103.90％(all RSDs≤5.50％).Conclusion The established qualitative method can rapidly,sensitively,and accurately identify the chemical constituents in YNO.The established quantitative method is sensitive,reliable,and specific,capable of comprehensively reflecting the contents of various components in YNO,thus providing a reference basis for quality evaluation and improvement of YNO.关键词
益脑膏/定性分析/含量测定/超高效液相色谱-串联质谱法Key words
Yinao Ointment(益 脑 膏)/Qualitative analysis/Content determination/Ultra-high performance liquid chromatography-tandem mass spectrometry分类
医药卫生引用本文复制引用
林隆,黄晓岚,吴珍琴,郑燕芳,黄鸣清,黄炜..基于UPLC-Q Exactive Plus Orbitrap MS和UPLC-MS的益脑膏化学成分定性和定量研究[J].时珍国医国药,2026,37(13):2464-2470,7.基金项目
国家自然科学基金(82274080) （82274080）
福建中医药大学附属福州中医院院内科研项目(2024-Ynky-09,2024-Ynky-08) （2024-Ynky-09,2024-Ynky-08）
福州市中医院重点专科培强建设项目-老年病科项目 （）
福建省科技创新联合资金项目(2025Y9594,2025Y9595) （2025Y9594,2025Y9595）
