时珍国医国药2026，Vol.37Issue(13)：2526-2533,8.DOI:10.70976/j.1008-0805.SZGYGY-2026-1319
枢经推拿对神经病理性疼痛大鼠脊髓神经元铁死亡的影响
Effect of pivot meridian Tuina on on ferroptosis of spinal cord neurons in rats with neuro-pathic pain
摘要
Abstract
Objective To investigate the effects of pivot meridian Tuina on ferroptosis in spinal neurons and the analgesic mechanisms in rats with neuropathic pain(neuropathic pain,NP)based on high mobility group box 1(HMGB1).Methods Sixty Sprague-Dawley(SD)rats were randomly divided into five groups:control,model,sham surgery,sham manipulation,and manipulation groups,with 12 rats in each group.Except for the control group,the model,sham manipulation,and manipulation groups were subjected to the L5 spinal nerve ligation(SNL)model,while the sham surgery group only exposed the spinal nerve without ligation.Interventions for the sham manipulation and manipulation groups began on the first day after surgery and continued for 14 days,with the other three groups receiving no intervention and observed for 14 days.Thermal withdrawal latency(PWL)and mechanical withdrawal threshold(PWT)were measured for all groups preoperatively and on postoperative days 1,3,7,10,and 14.After 14 days of intervention,the content of Fe2+,malondialdehyde(MDA),and glutathione(GSH)in the spinal cord was detected using the micromethod.Western blot(WB)and qPCR were used to assess the expression of HMGB1,glutathione peroxidase 4(GPX4),and anti-apoptotic protein Bcl-2-like protein 4(ACSL4)proteins and mRNA in the L4-L6 spinal cord of rats.Immunofluorescence(IF)was employed to observe the colocalization of HMGB1,4-hydroxynonenal(4-HNE),and neuronal nuclei(NeuN).Results Behavioral results indicated no statistically significant dif-ferences in PWL and PWT among all groups preoperatively(P>0.05);on the third postoperative day,PWL and PWT in the model,sham manipulation,and manipulation groups were significantly lower than those in the control and sham surgery groups(P<0.01),while PWL and PWT in the manipulation group began to be significantly higher than those in the model and sham manipulation groups from the tenth day(P<0.01).Micromethod detection results suggested that compared with the control and sham surgery groups,Fe2+and MDA content in the model group were significantly increased(P<0.01),while GSH content was significantly decreased(P<0.01),and manipulation intervention reversed this trend(P<0.01).WB and qPCR results indicated that the expression of HMGB1 and ASCL4 proteins and mRNA in the spinal cord of rats in the model and sham manipulation groups were significantly increased compared to the control and sham surgery groups(P<0.01),while GPX4 protein and mRNA expression were significantly decreased(P<0.01).Com-pared with the model and sham manipulation groups,manipulation intervention significantly reduced the expression of HMGB1 and ASCL4 proteins and mRNA(P<0.01)and increased GPX4 protein and mRNA expression(P<0.01).IF results suggested that com-pared with the control and sham surgery groups,HMGB1 and 4-HNE were significantly coexpressed with NeuN in the model group(P<0.01),and this relationship was reversed after manipulation intervention(P<0.01).Conclusion The analgesic mechanism of manipula-tion may be related to the downregulation of HMGB1 expression to inhibit ferroptosis in spinal neurons.关键词
枢经推拿/神经病理性疼痛/HMGB1/铁死亡/镇痛Key words
Pivot meridian Tuina/Neuropathic pain/HMGB1/Ferroptosis/Analgesia分类
医药卫生引用本文复制引用
王开龙,谭良源,甘小凤,符维艳,卢栋明,梁英业,甘振宝,胡跃强..枢经推拿对神经病理性疼痛大鼠脊髓神经元铁死亡的影响[J].时珍国医国药,2026,37(13):2526-2533,8.基金项目
国家自然科学基金(82260961,82460980) （82260961,82460980）
广西中医药大学自然科学基金(2024QN028) （2024QN028）
