天津中医药大学学报2026，Vol.45Issue(6)：689-699,11.DOI:10.11656/j.issn.1673-9043.2026.06.09
基于肠道菌群初步探讨半夏秫米汤改善失眠的作用机制
Preliminary exploration of the mechanism by which Banxia Shumi Decoction improves insomnia based on the gut microbiota
摘要
Abstract
[Objective]To investigate the effects of Banxia Shumi decoction on the gut microbiota in a mouse model of insomnia using 16S rRNA sequencing,and to preliminarily explore its underlying mechanisms.[Methods]60 SPF-grade female C57BL/6JNifdc mice were randomly divided into a blank group,a model group,a positive drug group,and low-,medium-,and high-dose Banxia Shumi decoction groups.Except for the blank group,mice in other groups received intraperitoneal injections of PCPA(400 mg/kg)combined with a water platform for three days to establish the model.After seven days of treatment,an open field test was conducted.Subsequently,fecal samples were collected for 16S rRNA sequencing to analyze the richness and species composition of the gut microbiota in mice.Immunofluorescence and Western blot were used to detect the expression levels of intestinal barrier proteins.Enzyme-linked immunosorbent assay was employed to measure the contents of 5-Hydroxytryptamine(5-HT)and Gamma-Aminobutyric Acid(GABA)in the hypothalamus and Interleukin-6(IL-6)in the colon.Hematoxylin and eosin(HE)staining was used to observe pathological damage in the mouse hypothalamus.Immunohistochemistry was applied to examine the expression of Ionized calcium-binding adapter molecule-1(Iba-1)in the mouse hypothalamus.[Results]Treatment with Banxia Shumi decoction increased the total distance traveled,average speed,and rearing frequency in mice,while reducing resting time.It also enhanced the richness and diversity of the gut microbiota,up-regulated the expression of intestinal barrier proteins,increased the contents of 5-HT and GABA in the hypothalamus,reduced colonic inflammation levels,alleviated pathological damage in the hypothalamus,and inhibited microglial activation.[Conclusion]Banxia Shumi decoction can alleviate pathological damage in the hypothalamus of mice with insomnia,exerting anti-insomnia effects by protecting the intestinal barrier,inhibiting inflammatory responses in the gut and brain,and modulating the gut microbiota.关键词
失眠/半夏秫米汤/肠道菌群/16S rRNA/肠屏障蛋白/炎症因子Key words
insomnia/Banxia Shumi Decoction/gut microbiota/16S rRNA/intestinal barrier proteins/inflammatory分类
医药卫生引用本文复制引用
王静,贾晓旭,张怡博,李非凡,朱金墙,郑文科..基于肠道菌群初步探讨半夏秫米汤改善失眠的作用机制[J].天津中医药大学学报,2026,45(6):689-699,11.基金项目
黑龙江省"揭榜挂帅"科技攻关项目(2023ZXJ02C01) （2023ZXJ02C01）
内蒙古自治区自然科学基金项目(2025MS08096) （2025MS08096）
天津市卫生健康委员会中医中西医结合科研课题项目(2023079). （2023079）
