天津中医药大学学报2026，Vol.45Issue(6)：700-708,9.DOI:10.11656/j.issn.1673-9043.2026.06.10
探讨芪麝丸及其有效成分延缓髓核细胞衰老的机制研究
Exploring the mechanism of Qishe Pills and its active component in delaying intervertebral disc degeneration and nucleus pulposus cell senescence:A study based on network pharmacology and experimental validation
摘要
Abstract
[Objective]To investigate the mechanism by which Qishe Pills(QSP)and its effective components delay intervertebral disc degeneration(IVDD)and nucleus pulposus(NP)cell senescence,utilizing network pharmacology and experimental validation.[Methods]Active components of QSP and their potential targets were screened based on network pharmacology,and a"Component-Target-Pathway"network was constructed.The binding affinity between active components of QSP and key targets of relevant signaling pathways was validated by molecular docking.Combined with in vitro experiments,an NP cell senescence model was established.Effective monomers were identified,and the upstream-downstream relationships of their action pathways were explored using Western blot and immunofluorescence assays.[Results]Network pharmacology revealed that the intersection targets were significantly enriched in the p53 signaling pathway and were associated with the PI3K/AKT,AMPK/SIRT1,ERK,and TGF-β/Smad pathways.Molecular docking and experimental validation identified tetrandrine as the effective component of QSP regulating IVDD.In vitro experiments confirmed that tetrandrine inhibits p53 pathway activity via the upstream PI3K/AKT/MDM2 pathway,thereby reducing NP cell apoptosis and promoting the synthesis of type II collagen(COL2A1).[Conclusion]NP cell senescence is a key pathological factor in IVDD.This study elucidates the mechanism by which QSP delay IVDD,identifies tetrandrine as its effective component,and demonstrates that tetrandrine mitigates IVDD and NP cell senescence by regulating the PI3K/AKT signaling pathway.关键词
椎间盘退变/芪麝丸/网络药理学/p53信号通路/体外实验Key words
intervertebral disc degeneration/Qishe Pills/network pharmacology/p53 signaling pathway/in vitro experiments分类
医药卫生引用本文复制引用
张程,王炳权,戴宇祥,王拥军,孙悦礼..探讨芪麝丸及其有效成分延缓髓核细胞衰老的机制研究[J].天津中医药大学学报,2026,45(6):700-708,9.基金项目
国家自然科学基金面上项目(82474536) （82474536）
上海市卫生健康委员会临床研究专项面上项目(202440110) （202440110）
2024年度江苏省中医药科技发展计划青年人才项目(QN202419) （QN202419）
2024年度苏州市应用基础研究科技创新项目(SYWD2024154) （SYWD2024154）
上海市第六人民医院院级科研基金项目(ynhg202420). （ynhg202420）
