天津中医药大学学报2026，Vol.45Issue(6)：709-716,8.DOI:10.11656/j.issn.1673-9043.2026.06.11
益肾化浊方调控CREB/BDNF通路改善学习记忆及神经元突触可塑性的机制研究
Based on the CREB/BDNF pathway:Investigation of the effects of the kidney-benefiting and turbidity-resolving formula on learning,memory,and neuronal synaptic plasticity
摘要
Abstract
[Objective]To investigate the effects of early intervention with the Yishen Huazuo Formula on learning and memory abilities,hippocampal synaptic structure and function,and the expression of proteins related to the cAMP response element-binding protein(CREB)/brain-derived neurotrophic factor(BDNF)pathway in SAMP8 mice,a model of accelerated aging.[Methods]Twenty-four 3-month-old male SAMP8 mice were randomly divided into a model(SAMP8)group and a Yishen Huazhuo Formula(YHD)group,with 12 mice in each group.Twelve male SAMR1 mice of the same age were selected as the control(SAMR1)group.The YHD group received oral administration of Yishen Huazhuo Formula granule solution,while the other two groups received the same dose of saline solution,administered once daily for 12 weeks.After administration,learning and memory abilities were evaluated using the Morris water maze test;Long-term potentiation(LTP)tests assessed synaptic neurophysiological function;Western blot analysis detected expression levels of proteins related to the"CREB/BDNF signaling pathway"in hippocampal tissue.[Results]Compared with the SAMP8 group,the YHD group showed significantly shorter escape latency times on days 4 and 5(P<0.05,P<0.01),with a trend toward increased platform crossings;The magnitude of LTP-induced fEPSP amplitude and slope enhancement was markedly elevated(P<0.05).Following TBS stimulation,the slope enhancement of LTP-induced fEPSP showed a significant increase(P<0.05).CREB protein expression was significantly increased(P<0.01),while BDNF and MecP2 protein expression showed an upward trend.p250GAP protein expression exhibited a downward trend without significant differences.[Conclusion]Early intervention with Yishen Huazuo Formula effectively improves learning and memory functions in SAMP8 mice.This may be related to its regulation of protein expression in the"CREB/BDNF signaling pathway,"improvement of neuronal ultrastructure,and subsequent enhancement of neuronal synaptic function.关键词
益肾化浊方/阿尔茨海默病/环磷腺苷效应元件结合蛋白/脑源性神经营养因子/突触可塑性Key words
Yishen Huazuo Formula/alzheimer's disease/cAMP response element-binding protein/brain-derived neurotrophic factor/synaptic plasticity分类
医药卫生引用本文复制引用
龚浩然,赵鑫伟,王凯,倪道艳,崔梦圆,刘政源,姜月,张琳琳..益肾化浊方调控CREB/BDNF通路改善学习记忆及神经元突触可塑性的机制研究[J].天津中医药大学学报,2026,45(6):709-716,8.基金项目
天津市卫生健康委员会中医中西医结合项目(2023025) （2023025）
国家自然科学基金青年基金项目(82104801) （82104801）
天津市教委科研计划项目(2023ZD035). （2023ZD035）
