塔里木大学学报2026，Vol.38Issue(3)：13-25,13.DOI:10.3969∕j.issn.1009-0568.2026.03.002
基于多组学技术的链霉菌5406促生防病功能分析
Functional analysis of growth promotion and disease control in Streptomyces alfalfae 5406 based on multi-omics
摘要
Abstract
Streptomyces alfalfae 5406,isolated from alfalfa rhizosphere soil,is a biocontrol strain with dual functions of plant growth promotion and disease control.This study aims to investigate the impact of tomato seedlings on the metabolites and gene expression of S.alfalfae 5406,identify growth-promoting and disease-resistant metabolites along with their regulatory genes,and assess the metabolite antibacterial activity against Ralstonia solanacearum.A co-culture system involving S.alfalfae 5406 and tomato seedlings was established.Integrated metabolomic and transcriptomic analyses were conducted to identify key differential metabolites and expressed genes.The functions of bioactive metabolites were validated through in vitro antibacterial assays.The results indicate that S.alfalfae 5406 is capable of synthesizing indole-3-acetic acid(IAA)through the tryptophan-dependent indole-3-acetamide(IAM)pathway.Additionally,it can produce gallic acid,methyl gallate,and daphnetin,all of which exhibited inhibitory activity against R.solanacearum.S.alfalfae 5406 demonstrates dual capabilities of promoting plant growth and suppressing diseases.It enhances plant growth and combats pathogens through various mechanisms,including IAA synthesis and bacteriostatic compound production.This research lays a groundwork for the potential development of agricultural biocontrol agents utilizing S.alfalfae 5406.关键词
链霉菌5406/促生活性/抑菌活性/多组学技术Key words
Streptomyces alfalfae 5406/growth-promoting activity/antimicrobial activity/multi-omics technologies分类
农业科技引用本文复制引用
郑玉华,李春强,张利莉..基于多组学技术的链霉菌5406促生防病功能分析[J].塔里木大学学报,2026,38(3):13-25,13.基金项目
海南省自然科学基金项目(325RC820) （325RC820）
