武汉工程大学学报2026，Vol.48Issue(3)：279-286,342,9.DOI:10.19843/j.cnki.CN42-1779/TQ.202508010
基于网络药理学探讨桂枝麻黄附子细辛汤治疗类风湿性关节炎的机制
Mechanism of Guizhi-Mahuang-Fuzi-Xixin Decoction for treating rheumatoid arthritis based on network pharmacology
摘要
Abstract
To explore the mechanism of Guizhi-Mahuang-Fuzi-Xixin Decoction in the treatment of rheumatoid arthritis(RA),network pharmacology and molecular docking technology were integrated.The active ingredients and corresponding targets of the decoction were screened via the TCMSP database.RA-related disease targets were retrieved from GeneCards and DrugBank databases,and the intersection of drug targets and disease targets was analyzed to lay the research foundation.Cytoscape 3.10.3 software was used to construct protein-protein interaction(PPI)networks and drug-active ingredient-target networks to screen key ingredients and core targets.GO functional annotation and KEGG pathway enrichment analysis were carried out on the Bioinformatics Cloud Platform,and molecular docking verification was performed using AutoDockTools software.Results showed that a total of 124 active ingredients were obtained,among which quercetin,luteolin and kaempferol were identified as key components.A total of 174 drug-disease core targets were screened,including TP53,TNF,AKT1,STAT3,ESR1,and HSP90AA1.GO functional enrichment acquired 6 513 entries,and KEGG pathway enrichment involved 197 pathways,mainly including inflammatory signaling pathways such as MAPK and IL-17,as well as pathways related to cancer,lipid metabolism,and atherosclerosis.Molecular docking results verified that naringenin possessed the lowest binding energy with ESR1.The decoction may exert therapeutic effects against RA by regulating the above core targets,participating in the inhibition of inflammatory factors and the regulation of signaling pathways.关键词
桂枝麻黄附子细辛汤/类风湿性关节炎/网络药理学/分子对接Key words
Guizhi-Mahuang-Fuzi-Xixin Decoction/rheumatoid arthritis/network pharmacology/molecular docking分类
医药卫生引用本文复制引用
向成加,向梅先..基于网络药理学探讨桂枝麻黄附子细辛汤治疗类风湿性关节炎的机制[J].武汉工程大学学报,2026,48(3):279-286,342,9.基金项目
湖北省高等学校实验室研究项目(SYYJ2025018) （SYYJ2025018）
