武警医学2026，Vol.37Issue(6)：497-505,9.DOI:10.3969/j.issn.1004-3594.2026.06.007
复杂作业环境心理应激阳性官兵血清微小RNA表达谱分析
Analysis of serum microRNA expression profile in military personnel with positive psycho-logical stress in complex operational environments
摘要
Abstract
Objective To investigate the differences in the expression profiles of microRNAs(miRNAs)in the serum of military personnel with positive psychological stress and the potential biomarkers.Methods A total of 100 young military personnel from a complex operational environment were randomly selected as the study subjects.The participants with positive psychological stress were identified using psychological assessments before and after the tasks,and their blood samples were collected accordingly.A total of 32 samples(16 pairs in total,comprising 8 pairs from the psychological stress negative group and 8 pairs from the psychological stress-positive group)were tested u-sing the GeneChip miRNA 4.0 array for the differences in miRNAs expression before and after the tasks.The downstream target genes of the differentially expressed miRNA were screened using miRanda and Targetscan databases.Kyoto Encyclopedia of Genes and Genomes(KEGG)was used to analyze the activated signaling pathways in the positive psychological stress group.The miRNAs with the most obvious differences were selected and verified by reverse transcription-quantitative polymerase chain reaction(RT-qPCR).Results A total of 22 miRNAs with significant differences were detected by gene chip detection.There were four miRNAs with the most obvious differences,among which the down-regulated miRNAs were hsa-miR-619-5p,hsa-miR-195-3p and hsa-miR-3196,and the up-regulated miRNAs was hsa-miR-378h.In ad-dition,64 019 differentially expressed downstream target genes were discovered.KEGG enrichment analysis showed that the transcription fac-tor BR-related signaling pathway was significantly activated in the positive psychological stress group.RT-qPCR results confirmed the most distinct miRNAs,and the results were consistent with the results of chip detection.Conclusions There are differences in the expression of miRNAs in the serum of military personnel with positive psychological stress,among which hsa-miR-619-5p,hsa-miR-195-3p,hsa-miR-3196 and hsa-miR-378h have high specificity and sensitivity,which may be used as biomarkers for positive psychological stress in military personnel.关键词
应激阳性/miRNA/特异性/生物标志物/复杂环境Key words
stress positive/miRNA/specificity/biomarker/complex environment分类
医药卫生引用本文复制引用
陈孝储,李娜,李正超,陈淑莲,陈旭义,王振国,苏彬,于溟璇,王仁杰..复杂作业环境心理应激阳性官兵血清微小RNA表达谱分析[J].武警医学,2026,37(6):497-505,9.基金项目
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