现代中西医结合杂志2026，Vol.35Issue(8)：1030-1036,7.DOI:10.3969/j.issn.1008-8849.2026.08.002
基于16S rRNA测序探究黄芪润肠汤对大肠黑变病肠道菌群的调控作用
Astragalus Decoction for moistening intestines treats melanosis coli via regulating intestinal flora based on 16S rRNA sequencing
摘要
Abstract
Objective It is to observe the effect of Astragalus Decoction for moistening intestines(ADMI)on intestinal mucosal injury and gut microbiota in rats with rhubarb-induced melanosis coli(MC),and to explore the mechanism of ac-tion of this drug in the treatment of MC.Methods Sixty SD rats were randomly divided into normal group,model group,low-dose and high-dose groups of ADMI,lactulose group,with 12 rats in each group.The rats of all groups except for the blank group were continuously fed with raw rhubarb solution via gastric administration for 30 days to establish models of MC.After successful modeling,the low-dose and high-dose groups of ADMI were administered ADMI via gastric gavage at the doses of 0.75 mL/100 g and1.5 mL/100 g,respectively,while the lactulose group was administered an oral lactulose solution at a dose of 1 g/kg,all once daily,continuously treated for 28 days.The gross appearance of colon tissue from rats in each group was examined,the histopathology of the colon tissue was observed by HE staining,and fecal samples were collected from each group for 16S rRNA sequencing analysis.Results Significant pigment deposition in colon tissue was ob-served in the rats of the model group;there was extensive shedding of colonic mucosal epithelial cells,and brown pigment deposits were clearly visible in the lamina propria and submucosa;compared with the model group,pigment deposition in colon tissue and brown pigment deposition in the lamina propria and submucosa of colon were reduced with minimal shed-ding of colonic mucosal epithelial cells in the rats of the medicated groups,and the improvements were more significant in the high-dose group of ADMI and lactulose group.Analysis of fecal alpha diversity showed that the colonic microbial abun-dance and diversity were significantly increased in the high-dose group of ADMI and lactulose group;PCA analysis of beta diversity revealed that the samples in the model group were clearly distinct from those in the other groups;species-level a-nalysis showed that the abundances of Akkermansia muciniphila,Phocaeicola massiliensis,Phocaeicola vulgatus,and Kineo-thrix alysoides were all increased to varying degrees in both the low-dose and high-dose groups of ADMI.Conclusion ADMI can treat MC via regulating intestinal flora.关键词
16S rRNA/肠道菌群/大肠黑变病/黄芪润肠汤Key words
16S rRNA/intestinal flora/melanosis coli/Astragalus Decoction for moistening intestines分类
医药卫生引用本文复制引用
喻少雷,孙凤娟,戴方冉,杨爱龙..基于16S rRNA测序探究黄芪润肠汤对大肠黑变病肠道菌群的调控作用[J].现代中西医结合杂志,2026,35(8):1030-1036,7.基金项目
黑龙江省中医药科研项目(ZHY2022-192) （ZHY2022-192）
