现代中西医结合杂志2026，Vol.35Issue(8)：1050-1055,6.DOI:10.3969/j.issn.1008-8849.2026.08.005
白藜芦醇以JUN为靶点促进成纤维细胞凋亡抑制瘢痕增生的作用研究
Resveratrol targeting JUN inhibits scarring via promoting fibroblast apoptosis
摘要
Abstract
Objective It is to investigate the mechanism of resveratrol targeting JUN in promoting fibroblast apoptosis and inhibiting scar hyperplasia through transcriptome sequencing and cell experiments.Methods ①Transcriptome sequen-cing and real-time fluorescence quantitative PCR were performed to confirm the differences in the expression levels of fibro-sis gene α-SMA,collagen type Ⅰ,pro-apoptotic gene Bcl-2 and JUN in human hypertrophic scar fibroblasts(HSF)and human dermal fibroblasts(DF).Quantitative fluorescent PCR was used to verify the differences in the mRNA expression levels of JUN in HSF and DF.②HSF cells were collected and divided into the following groups:a control group,a 2 μmol/L resveratrol group,a 10 μmol/L resveratrol group,a 50 μmol/L resveratrol group,and a curcumin A+50 μmol/L resvera-trol group.The mRNA expressions of JUN,Col-Ⅰ,and α-SMA in the cells of the first four groups,and the mRNA expres-sions of p53,Caspase-3,Caspase-9,protein kinase B(Akt),and mammalian target of rapamycin(mTOR)in the cells of all the five groups were detected by RT-qPCR,the protein expressions of Col-Ⅰ and α-SMA in the cells of the first four groups were detected by Western Blot method.Results ①The relative mRNA expressions of JUN,Col-Ⅰ,α-SMA,and Bcl-2 in HSF were significantly higher than those in DF(all P<0.05).②The relative mRNA expressions of JUN,Col-Ⅰ,α-SMA,Akt,and mTOR,as well as the relative protein expressions of Col-Ⅰ and α-SMA in HSF of the 10 μmol/L resveratrol group and 50 μmol/L resveratrol group were significantly lower than those in HSF of the control group(all P<0.05),while the relative mRNA expressions of p53,Caspase-3,and Caspase-9 in HSF of the 10 μmol/L resveratrol group and 50 μmol/L resveratrol group were significantly higher than those in HSF of the control group(all P<0.05),there were no significant differences in the relative mRNA expressions of p53,Caspase-3,Caspase-9,Akt and mTOR between the curcumin A+50 μmol/L resveratrol group and blank group(all P>0.05).Conclusion Resveratrol targeting JUN can promote the apoptosis of fibroblasts and inhibit the occurrence and development of hypertrophic scar.关键词
白藜芦醇/增生性瘢痕/成纤维细胞/JUN/皮肤纤维化/凋亡Key words
resveratrol/hypertrophic scar/fibroblast/JUN/skin fibrosis/apoptosis分类
医药卫生引用本文复制引用
孙子荔,刘晨阳,孟凡胜,皇苏州,袁冰峰,李震,徐超,吴丽,花海兵..白藜芦醇以JUN为靶点促进成纤维细胞凋亡抑制瘢痕增生的作用研究[J].现代中西医结合杂志,2026,35(8):1050-1055,6.基金项目
国家自然科学基金资助项目(82305238) （82305238）
江阴市中医药学会科研项目(Z202203) （Z202203）
"太湖之光"科技攻关项目(K20221025) （K20221025）
无锡市"双百"中青年医疗卫生拔尖人才(HB2023052) （HB2023052）
